Multilayered oriented polyfluorene (PF) films were obtained by applying thermal treatment procedure to a multilayered PF film constructed with fluorene derivatives layer formed on top of a highly oriented friction-transferred crystalline poly(9,9-dioctylfluorene) (PF8) film. The orientations in the multilayered PF films were investigated by polarized photoluminescence (PL) spectroscopy and grazing incident X-ray diffraction (GIXD) analysis. The results of the multilayered PF film constructed with spin-coated PF8 on friction-transferred PF8 indicate that the rearrangement of the upper PF8 layer is induced from the orientation of lower PF8 layer by thermal treatment at the nematic phase temperature. Polarized green emission from the multilayered oriented PF film was demonstrated using the blend of PF8 and poly(9,9-dioctylfluorene-co-benzothiadiazol) (F8BT) as green light emitter for upper layer. By this method, the polarized emission color can be tuned using polymer blends for Upper layer similar to the liquid-crystalline polymer arrangement without using different materials as an underlying layer such as the rubbed polyimide.