The responses of proteins to different forms of stress are not well understood at the present time. The "nonthermal" effect of electric field oil proteins has recently become an area of great theoretical and practical interest. Possible effects Of Pulsed electric field oil proteins have been predicted by theoretical simulations. However, there is still a lack of experimental reports. Ill this work, experimental Studies were carried out to investigate and compare the effects of 3.5 x 10(6) V/m pulsed electric field and thermal stresses on the conformational changes of lysozyme in Solution. The changes in secondary Structure and conformation of disulfide linkage (S-S) of lysozyme were investigated experimentally by circular dichroism analysis and micro-Raman spectra. The motions of tryptophan and tyrosine side chains in lysozyme were also evaluated. From the experimental data, different actions of electric field and thermal stresses oil the protein were discovered.