In this work we present an analysis of the mass transport in the anode side Porous backing layer of a direct methanol fuel cell (DMFC). The effective transport coefficient of different backing layers at various compressions was measured and compared to two different literature models and a single particle random walk simulation which accounts for details of the geometrical fibre microstructure. Based on the measured values of the effective transport coefficient limiting Current densities for diffusive transport were calculated taking into account geometric boundary conditions and anisotropic and inhomogeneous backing layer properties. Comparison with the measured values for the limiting Current in fuel cell operation shows qualitative agreement. A systematic underestimation indicates that also other transport processes contribute significantly to the mass transfer at the used experimental setup. (C) 2009 Elsevier B.V. All rights reserved.