Double-quantum C-13 nuclear magnetic resonance of bathorhodopsin, the first photointermediate in mammalian vision

Concistre M; Gansmuller A; McLean N; Johannessen OG; Montesinos IM; Bovee-Geurts PHM; Verdegem P; Lugtenburg J; Brown RCD; DeGrip WJ; Levitt MH

Journal of the American Chemical Society, Vol.130, No.32, 10490-10490, 2008

Concistre M, Gansmuller A, McLean N, Johannessen OG, Montesinos IM, Bovee-Geurts PHM, Verdegem P, Lugtenburg J, Brown RCD, DeGrip WJ, Levitt MH

The C-13 chemical shifts of the primary visual photointermediate bathorhodopsin have been observed by performing double-quantum magic-angle-spinning NMR at low temperature in the presence of illumination. Strong isomerization shifts have been observed upon the conversion of rhodopsin into bathorhodopsin.