Electrogenerated chemiluminescence (ECL) can be obtained from an etectrochemically oxidized highly oriented pyrolytic graphite electrode or a graphene oxide suspension in an aqueous solution containing 0.1 M NaClO4, phosphate buffer (PBS) (pH = 7.0) and 13 mM tri-n-propytamine. Single ECL events from individual graphene oxide nanosheets can be observed. The observation of ECL in such systems may lead to the development of a new class of carbon-based nanomateriats for potentially highly sensitive analytical applications.