In this Communication, we report one-pot, high yield formation of unprecedented 1-D necklace-like chains consisting of hybrid Pd nanocrystals (NCs) embedded in spheres of a peptidic lipid. We employed the synthetic peptidic Lipid, 2-(2-tetradecanamidoacetamido)acetic acid (1). We found that in the presence of palladium acetate [Pd(OAc)(2)] 1 can self-assemble into structured spheres which connect each other one by one to form the necklace chains in ethanol solutions by coordination of negatively charged carboxylic (COO-) groups with Pd-II. Upon reduction of ethanol, the coordinated Pd-II are reduced into Pd atoms, which subsequently initiate the generation of Pd nuclei, and finally grow into Pd NCs embedded in the sphere chain of 1.