화학공학소재연구정보센터
Journal of the American Chemical Society, Vol.131, No.20, 6952-6952, 2009
Atomic-Level Mapping of Antibody Epitopes on a GPCR
Epitopes that define the immunodominant regions of conformationally complex integral membrane proteins have been difficult to reliably delineate. Here, a high-throughput approach termed shotgun mutagenesis was used to map the binding epitopes of five different monoclonal antibodies targeting the GPCR CCR5. The amino acids, and in some cases the atoms, that comprise the critical contact points of each epitope were identified, defining the immunodominant structures of this GPCR and their physicochemistry.