The power of slit-flow ektacytometry measurements for testing normal and heat treated red blood cells using various viscosity media in laboratory animals

Ferenc Kiss; Erika Sajtos; Timea Hever; Norbert Nemeth

Korea-Australia Rheology Journal, Vol.22, No.1, 81-86, March, 2010

Ferenc Kiss, Erika Sajtos, Timea Hever, and Norbert Nemeth^†

Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, Universityof Debrecen, Hungary

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Red blood cell (RBC) deformability values resulted from ektacytometry tests can be influenced by the viscosity of the medium in which the RBCs are suspended for measurement. To determine the power of measurements using various viscosity media in this study we used normal and heat treated RBCs from laboratory rats and beagle dogs. A RheoScan-D200 slit-flow ektacytometer was used to measure RBC deformability. Blood samples were taken from female Sprague-Dawley rats and inbred beagle dogs. Before and after heat treatment of RBC suspensions ektacytometry tests were performed using PVP solutions at viscosity of 15, 20 and 30 mPa.s. Heat treatment caused impaired RBC deformability in both species and in every PVP solution. The difference between normal and heat treated RBCs was the highest in rats, while in dogs the magnitude of change was smaller, however being significant. Well comparable and stable results were found using 30 mPa.s media. The sensitivity of RBCs for heat treatment seems to be higher in rats. The suspending PVP medium at 30 mPa.s is recommended for testing RBC deformability by ektacytometry in laboratory rats and dogs, too, because this media resulted in the most stable data when comparing normal and rigid cells.

Keywords:red blood cell deformability;ektacytometry;suspending medium viscosity;heat treatment;power of measurement;hemorheology

[References]

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Baskurt OK, Hardeman MR, Uyuklu M, Ulker P, Cengiz M, Nemeth N, Shin S, Alexy T, Meiselman HJ, Biorheology, 46, 251 (2009)
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Fisher TM, Biophys. J., 93, 2553 (2007)
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Musielak, M, Clin. Hemorheol. Microcirc., 42, 47 (2009)
Nash GB, Meiselman HJ, Biorheology, 22, 73 (1985)
Nemeth N, Alexy T, Furka A, Baskurt OK, Meiselman HJ, Furka I, Miko I, Biorheology, 46, 155 (2009)
Nemeth N, Baskurt OK, Meiselman HJ, Kiss F, Uyuklu M, Hever T, Sajtos E, Kenyeres P, Toth K, Furka I, Miko I, Korea-Aust. Rheol. J., 21(2), 127 (2009)
Nemeth N, Baskurt OK, Meiselman HJ, Furka I, Miko I, Korea-Aust. Rheol. J., 21(3), 155 (2009)
Nemeth N, Baskurt OK, Meiselman HJ, Miko I, Clin. Hemorheol. Microcirc., 43, 257 (2009)
Nemeth N, Kiss F, Sajtos E, Matyas L, Magyar Z, Furka I, Miko I, Korea-Aust. Rheol. J., - submitted. (2010)
Pfafferott C, Nash GB, Meiselman HJ, Biophys. J., 47, 695 (1985)
Repin NV, Bobrova EN, Repina SV, Bioelectrochemistry, 73, 101 (2008)
Shin S, Ku Y, Park MS, Suh JS, Cytometry Part B (Clinical Cytometry), 65, 6 (2005)
Stuart J, Stone PCW, Freyburger G, Boisseau MR, Altman DG, Clin. Hemorheol., 9, 181 (1989)
Windberger U, Baskurt OK, Comparative hemorheology, in: Handbook of Hemorheology and Hemodynamics, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ, eds, IOS Press, Amsterdam, The Netherlands, pp. 267-285 (2007)

[1] Baskurt OK, Boynard M, Cokelet GC, Connes P, Cooke BM, Forconi S, Liao F, Hardeman MR, Jung F, Meiselman HJ, Nash G, Nemeth N, Neu B, Sandhagen B, Shin S, Thurston G, Wautier JL, Clin. Hemorheol. Microcirc., 42, 75 (2009)

[2] Baskurt OK, Hardeman MR, Uyuklu M, Ulker P, Cengiz M, Nemeth N, Shin S, Alexy T, Meiselman HJ, Biorheology, 46, 251 (2009)

[3] Baskurt OK, Meiselman HJ, Clin. Hemorheol. Microcirc., 31, 23 (2004)

[4] Fisher TM, Biophys. J., 93, 2553 (2007)

[5] Fisher T, Schmid-Schonbein H, Tank tread motion of red cell membranes in viscometric flow, in: Red Cell Rheology, Bessis M, Shohet SB, Mohandas N, eds, Springer Verlag, Berlin, pp. 347 -358. (1978)

[6] Foo JJ, Chan V, Feng ZQ, Liu KK, Biomed. Mater., 1, 1 (2006)

[7] Hardeman MR, Personal communication.

[8] Hardeman MR, Goedhart PT, Shin S, Methods in hemorheology, in: Handbook of Hemorheology and Hemodynamics, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ, eds, IOS Press, Amsterdam, The Netherlands, pp. 242-266. (2007)

[9] Musielak, M, Clin. Hemorheol. Microcirc., 42, 47 (2009)

[10] Nash GB, Meiselman HJ, Biorheology, 22, 73 (1985)

[11] Nemeth N, Alexy T, Furka A, Baskurt OK, Meiselman HJ, Furka I, Miko I, Biorheology, 46, 155 (2009)

[12] Nemeth N, Baskurt OK, Meiselman HJ, Kiss F, Uyuklu M, Hever T, Sajtos E, Kenyeres P, Toth K, Furka I, Miko I, Korea-Aust. Rheol. J., 21(2), 127 (2009)

[13] Nemeth N, Baskurt OK, Meiselman HJ, Furka I, Miko I, Korea-Aust. Rheol. J., 21(3), 155 (2009)

[14] Nemeth N, Baskurt OK, Meiselman HJ, Miko I, Clin. Hemorheol. Microcirc., 43, 257 (2009)

[15] Nemeth N, Kiss F, Sajtos E, Matyas L, Magyar Z, Furka I, Miko I, Korea-Aust. Rheol. J., - submitted. (2010)

[16] Pfafferott C, Nash GB, Meiselman HJ, Biophys. J., 47, 695 (1985)

[17] Repin NV, Bobrova EN, Repina SV, Bioelectrochemistry, 73, 101 (2008)

[18] Shin S, Ku Y, Park MS, Suh JS, Cytometry Part B (Clinical Cytometry), 65, 6 (2005)

[19] Stuart J, Stone PCW, Freyburger G, Boisseau MR, Altman DG, Clin. Hemorheol., 9, 181 (1989)

[20] Windberger U, Baskurt OK, Comparative hemorheology, in: Handbook of Hemorheology and Hemodynamics, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ, eds, IOS Press, Amsterdam, The Netherlands, pp. 267-285 (2007)