Interaction between cells and extracellular matrix and mechanical properties of cell surfaces are discussed. Using atomic force microscope (AFM), we observed two different kinds of human ovary carcinoma cells (with highly invasive and minimally invasive ability) and their surrounding fibronectin fibrils. The adhesive properties of both cells are investigated using the AFM force-displacement technique. Results show in the following. The shape of cells that adhered to the glass slide is rectangular. Sucker-shape structures exist at both ends of the cell. Around cells with highly invasive ability, there are fewer fibrils and their length is short; however, the surface has a higher adhesion force. The adhesion forces at the middle and end parts of cells with highly invasive ability are about 120(-25)(+20) and 210(-35)(+50) nN, respectively. Around cells with minimally invasive ability are more fibrils and their length is long; however, the surface has a smaller adhesion force. The adhesion forces at the middle and end parts of cells with minimally invasive ability are about 75(-11)(+17) and 105(-30)(+25) nN, respectively. Moreover, on the surface of both cells, the adhesion force at the end of the cell (similar to acetabulum) is greater than that at the middle of the cell. These conclusions agree well with biological functions and properties of both cells.