The adsorption kinetics of extruded 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC)/1-(cis-9-octadecenoyl)rac-glycerol (monoolein, MO) aggregates on SiO2 surface at 25 degrees C is investigated in real time, using the dissipative quartz crystal microbalance (QCM) technique. Four adsorption pathways have been identified depending on the molar fraction of MO in the DPPC/MO system: (I) intact vesicle adsorption, (II) vesicle reorganization on a SiO2 surface, (III) supported lipid bilayer (SLB) formation, and (IV) cubosome adsorption. The results can be understood by the fact that DPPC is a lamellar phase-forming lipid, whereas MO prefers the cubic phase. Therefore, the incorporation of MO in DPPC increases the packing parameter. Equally important, MO also increases the mobility of lipid molecules and lateral pressure in the bilayers as a result of the presence of a unique cis- double bond. Before extrusion, the vesicles size increases with the MO content when X-MO <= 0.7 and cubosomes are formed for X-MO >= 0.8. The extruded DPPC/MO suspensions consist of reformed vesicles for X-MO <= 0.7 and filtered cubosomes for X-MO >= 0.8, all with a uniform diameter of similar to 100 nm. Differential scanning calorimetry (DSC) further indicates that the addition of MO lowers the main phase transition temperature of DPPC and thus makes the hydrophobic interior more fluid.