In this paper we explored factors that determine the polarity of an Actin bundle formed with polycation through electrostatic interaction. We found that the polarity decreases with an increase in the polycation concentration while it hardly depends on the KCl salt concentration. Additionally, the polarity of the Actin bundle increases with an increase in the degree of polymerization of the polycation at a constant polymer concentration. From these results we proposed that the kinetics of nuclei formation determines the polarity of the Actin bundle.