Contrast between hydrophilic and hydrophobic domains and probe-sample adhesion forces as a function of relative humidity (RH) and sample surface free energy have been investigated using hydrophilic and hydrophobic atomic force microscopy (AFM) probes. For hydrophobic probes, the adhesion force is low, and the AFM image contrast between hydrophilic and hydrophobic domains is poor over the 0-93% RH. For hydrophilic probes, the image contrast between the hydrophilic and hydrophobic domains is poor at low RH but improved at high RH. This image contrast change is related to adhesion force differences between the two domains. In turn, the enhanced adhesion and image contrasts at elevated RH are attributed to capillary forces, which are large over the hydrophilic domains but greatly diminished over the hydrophobic domains. The adhesion force increases slightly with sample surface free energy at low RH, but increases rapidly with increasing sample surface free energy at high RH. The results indicate that for AFM in air, tailoring the RH of the probe-sample environment and utilizing a hydrophilic probe can enhance imaging of materials chemical heterogeneity with nanoscale spatial resolution.