Preparation of bacteria microarray using selective patterning of polyelectrolyte multilayer and poly(ethylene glycol)-poly(lactide) diblock copolymer

Chang-Hyung Choi; Ji-Hye Lee; Taek-Sung Hwang; Chang-Soo Lee; Yun-Gon Kim; Yung-Hun Yang; Kang Moo Huh

Macromolecular Research, Vol.18, No.3, 254-259, March, 2010

DOI10.1007/s13233-010-0314-6 Export Citation

Preparation of bacteria microarray using selective patterning of polyelectrolyte multilayer and poly(ethylene glycol)-poly(lactide) diblock copolymer

Chang-Hyung Choi, Ji-Hye Lee, Taek-Sung Hwang , Chang-Soo Lee^†, Yun-Gon Kim¹, Yung-Hun Yang², and Kang Moo Huh³

Department of Chemical Engineering, Chungnam National University, Daejeon, 305-764, Korea
¹Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA
²Department of Microbial Engineering, Konkuk University, Seoul, 143-701, Korea
³Department of Polymer Science and Engineering, Chungnam National University, Daejeon, 305-764, Korea

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This study reports a simple but efficient method for create bacteria microarrays on a predesigned functional surface consisting of two distinctive regions; a bacterial immobilizing area and a nonimmobilizing region. The functionalized surface was fabricated by a combination of self-assembled polyelectrolyte multilayers (PEL) and micromolding in the capillaries (MIMIC) of poly(ethylene glycol)-poly(d,l-lactide) diblock copolymer (PEGPLA). The PEL region provides bacterial immobilization, and the nonspecific binding of bacteria was prevented using PEG-PLA as passivation molecules. The topological change in the functionalized surface was characterized by atomic force microscopy (AFM), which suggested the occurrence of a laterally homogeneous and well defined surface modification. An analysis of the fluorescence signals from the bacteria microarray indicates that the bacteria are viable and grow after immobilization on patterned surface features. The rapid fabrication of two different functionalities on the surface results in a uniform bacteria pattern with a high signal to noise ratio and shape flexibility, such as lines, squares, and circles. Moreover, the pattern size could be controlled from 20 to 100 mu m.

Keywords:poly(ethylene glycol)-poly(actide) copolymer (PEG-PLA);polyelectrolyte multilayers (PEL);micromolding in capillaries (MIMIC);bacteria;microarrays

[References]

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Lee CS, Lee SH, Kim YG, Lee JH, Kim YK, Kim BG, Biosens. Bioelectron., 22, 891 (2007)
Zhu H, Snyder M, Curr. Opin. Chem. Biol., 5, 40 (2001)
Lee WJ, Choi DK, Lee Y, Kim DN, Park JW, Koh WG, Sens. Actuators B-Chem., 129, 841 (2008)
Kim SK, Kim BG, Lee JH, Lee CS, Macromol. Res., 17(4), 232 (2009)
Ginger DS, Zhang H, Mirkin CA, Angew. Chem.-Int. Edit., 43, 30 (2004)
Millau JF, Raffin AL, Caillat S, Claudet C, Arras G, Ugolin N, Douki T, Ravanat JL, Breton J, Oddos T, Dumontet C, Sarasin A, Chevillard S, Favier A, Sauvaigo S, Lab on a Chip, 8, 1713 (2008)
Fernandes TG, Kwon SJ, Lee MY, Clark DS, Cabral JMS, Dordick JS, Anal. Chem., 80, 6633 (2008)
Bahlis N, Stewart D, Shin B, Mansoor A, Blood, 110, 138 (2007)
Rozhok S, Fan ZF, Nyamjav D, Liu C, Mirkin CA, Holz RC, Langmuir, 22(26), 11251 (2006)
Thirumalapura NR, Ramachandran A, Morton RJ, Malayer JR, J. Immunol. Methods, 309, 48 (2006)
Xu LP, Robert L, Qi OY, Taddei F, Chen Y, Lindner AB, Baigl D, Nano Lett., 7, 2068 (2007)
Shim HW, Lee JW, Lee CS, Korean J. Biotechnol. Bioeng., 4, 325 (2006)
Moskovits G, Moseley FP, J. Bacteriol., 88, 1815 (1964)
Weibel DB, Lee A, Mayer M, Brady SF, Bruzewicz D, Yang J, DiLuzio WR, Clardy J, Whitesides GM, Langmuir, 21(14), 6436 (2005)
Cho J, Char K, Hong JD, Lee KB, Adv. Mater., 13(14), 1076 (2001)
Cho J, Jang H, Yeom B, Kim H, Kim R, Kim S, Char K, Caruso F, Langmuir, 22(3), 1356 (2006)
Lee JH, Kim HE, Im JH, Bae YM, Choi JS, Huh KM, Lee CS, Colloids Surf. B: Biointerfaces, 64, 126 (2008)
Elbert DL, Hubbell JA, Chem. Biol., 5, 177 (1998)
Krishnan S, Weinman CJ, Ober CK, J. Mater. Chem., 18, 3405 (2008)
Shim HW, Lee JH, Hwang TS, Rhee YW, Bae YM, Choi JS, Han J, Lee CS, Biosens. Bioelectron., 22, 3188 (2007)
Shim HW, Lee JH, Kim BY, Son YA, Lee CS, J. Nanosci. Nanotechnol., 9, 1204 (2009)
Haynie DT, Zhang L, Rudra JS, Zhao WH, Zhong Y, Palath N, Biomacromolecules, 6(6), 2895 (2005)
Bucior K, Fischer J, Patrykiejew A, Tscheliessnig R, Sokolowski S, J. Chem. Phys., 126, 9 (2007)
Rauf S, Zhou DJ, Abell C, Klenerman D, Kang DJ, Chem. Commun., 1721 (2006)
Calvo EJ, Battaglini F, Danilowicz C, Wolosiuk A, Otero M, Faraday Discuss., 116, 47 (2000)
Abbasnezhad H, Gray MR, Foght JM, Colloids Surf. B: Biointerfaces, 62, 36 (2008)
Gallardo-Moreno AM, Gonzalez-Martin ML, Bruque JM, Perez-Giraldo C, Sanchez-Silos R, Gomez-Garcia AC, J. Adhes. Sci. Technol., 17(14), 1877 (2003)
Zhou XC, Zhou JZ, Proteomics, 6, 1415 (2006)

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[3] Zhu H, Snyder M, Curr. Opin. Chem. Biol., 5, 40 (2001)

[4] Lee WJ, Choi DK, Lee Y, Kim DN, Park JW, Koh WG, Sens. Actuators B-Chem., 129, 841 (2008)

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[8] Fernandes TG, Kwon SJ, Lee MY, Clark DS, Cabral JMS, Dordick JS, Anal. Chem., 80, 6633 (2008)

[9] Bahlis N, Stewart D, Shin B, Mansoor A, Blood, 110, 138 (2007)

[10] Rozhok S, Fan ZF, Nyamjav D, Liu C, Mirkin CA, Holz RC, Langmuir, 22(26), 11251 (2006)

[11] Thirumalapura NR, Ramachandran A, Morton RJ, Malayer JR, J. Immunol. Methods, 309, 48 (2006)

[12] Xu LP, Robert L, Qi OY, Taddei F, Chen Y, Lindner AB, Baigl D, Nano Lett., 7, 2068 (2007)

[13] Shim HW, Lee JW, Lee CS, Korean J. Biotechnol. Bioeng., 4, 325 (2006)

[14] Moskovits G, Moseley FP, J. Bacteriol., 88, 1815 (1964)

[15] Weibel DB, Lee A, Mayer M, Brady SF, Bruzewicz D, Yang J, DiLuzio WR, Clardy J, Whitesides GM, Langmuir, 21(14), 6436 (2005)

[16] Cho J, Char K, Hong JD, Lee KB, Adv. Mater., 13(14), 1076 (2001)

[17] Cho J, Jang H, Yeom B, Kim H, Kim R, Kim S, Char K, Caruso F, Langmuir, 22(3), 1356 (2006)

[18] Lee JH, Kim HE, Im JH, Bae YM, Choi JS, Huh KM, Lee CS, Colloids Surf. B: Biointerfaces, 64, 126 (2008)

[19] Elbert DL, Hubbell JA, Chem. Biol., 5, 177 (1998)

[20] Krishnan S, Weinman CJ, Ober CK, J. Mater. Chem., 18, 3405 (2008)

[21] Shim HW, Lee JH, Hwang TS, Rhee YW, Bae YM, Choi JS, Han J, Lee CS, Biosens. Bioelectron., 22, 3188 (2007)

[22] Shim HW, Lee JH, Kim BY, Son YA, Lee CS, J. Nanosci. Nanotechnol., 9, 1204 (2009)

[23] Haynie DT, Zhang L, Rudra JS, Zhao WH, Zhong Y, Palath N, Biomacromolecules, 6(6), 2895 (2005)

[24] Bucior K, Fischer J, Patrykiejew A, Tscheliessnig R, Sokolowski S, J. Chem. Phys., 126, 9 (2007)

[25] Rauf S, Zhou DJ, Abell C, Klenerman D, Kang DJ, Chem. Commun., 1721 (2006)

[26] Calvo EJ, Battaglini F, Danilowicz C, Wolosiuk A, Otero M, Faraday Discuss., 116, 47 (2000)

[27] Abbasnezhad H, Gray MR, Foght JM, Colloids Surf. B: Biointerfaces, 62, 36 (2008)

[28] Gallardo-Moreno AM, Gonzalez-Martin ML, Bruque JM, Perez-Giraldo C, Sanchez-Silos R, Gomez-Garcia AC, J. Adhes. Sci. Technol., 17(14), 1877 (2003)

[29] Zhou XC, Zhou JZ, Proteomics, 6, 1415 (2006)