Structural, optical, and photocatalytic properties of various TiO2 nanostructures prepared by glancing angle deposition (GLAD) and by electrochemical anodic oxidation of Ti have been studied. The TiO2 nanorods were prepared on unheated glass substrates by using reactive sputtering of Ti in the GLAD regime. TiO2 nanotubes and brush-type nanostructures were fabricated by anodic oxidation of flat Ti films and Ti nanorods prepared by GLAD, respectively. The optical studies revealed that the nanotubes and brush-type nanostructures possess antireflection properties. The photocatalytic activity of TiO2 nanostructures was characterized by following decomposition of isopropanol under visible and UV light irradiation and found to be significantly higher in nanostructured samples than in their flat counterparts. Also, TiO2 nanotubes and brush-type nanostructures showed superior photocatalytic activity in comparison with nanorods due to a significantly higher specific surface area.