Oxygen/water vapor-plasma treated polished aluminum substrates were coated with poly(acrylic acid) (PAA) using deeping-coating technique, and subsequently heated. ESCA, chemical derivatization and SEM confirmed the successful coating of substrates. It was shown that the covalently attached PAA macromolecules exhibit anti-bacterial characteristics. Samples coated with PAA exposed to a 5 strain mixture of Listeria monocytogenes for 24 hours resulted in 98% decrease in the bacterial population. A mix of three different bacteria, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Escherichia coli was also tested. A reduction of 82 to 96% of bacterial numbers was obtained. Experimental results indicated that double layer structures could also be prepared from PAA-coated surfaces. PAA and poly(vinylpyridine) (PVP) double layers were successfully generated.