New Top Coating System of Chemically Anchored Phospholipid Monolayer New Top Coating System of Chemically Anchored Phospholipid Monolayer

Kwangmeyung Kim; Sangjin Park; Youngro Byun; Hyun Tae Moon

Macromolecular Research, Vol.18, No.5, 519-525, May, 2010

DOI10.1007/s13233-010-0515-z Export Citation

New Top Coating System of Chemically Anchored Phospholipid Monolayer New Top Coating System of Chemically Anchored Phospholipid Monolayer

Kwangmeyung Kim , Sangjin Park, Youngro Byun¹, and Hyun Tae Moon^{2, †}

Biomedical Research Center, Korea Institute of Science and Technology, Seoul, 136-719, Korea
¹Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Pharmacy, Seoul National University, Seoul, 151-742, Korea
²Mediplex Corp. Korea, Seoul, 152-790, Korea

E-mail:

We report a novel method for preparing multi-layer polymer films as a matrix for local drug delivery that can be used as a drug-eluting stent. The base polymer matrix was an acrylated polymer film (poly(octadecyl acrylateco-hydroxybutyl acrylate, poly(OA-co-HA)), where echinomycin (EM) was dispersed into the poly(OA-co-HA) matrix. The top coating layer consisted of a chemically grafted phospholipid polymeric monolayer (polyPC) onto the acrylated polymer film. The chemically grafted phospholipid monolayer was prepared using in situ photopolymerization of a pre-assembled acrylated phospholipid (1-stearoyl-2-[12-(acryloyloxy)-dodecanoyl]-sn-glycero-3-phosphocholine) monolayer, produced by lipid vesicle fusion, onto the acrylated polymer film. Physiochemical characterization of the drug-encapsulated multi-layer polymer films was performed using scanning electron microscopy (SEM), water contact angle measurements and X-ray photon spectroscopy (XPS). Compared to the bare acrylated polymer film, the chemically grafted phospholipid monolayer over the acrylated polymer film showed significant changes in both the surface chemistry and drug release pattern. This phospholipid modified multi-layer polymer system was capable of reducing the burst release of the drug. In addition, it inhibited platelet adhesion and smooth muscle cell proliferation, in vitro, due to the highly dense phospholipid monolayer.

Keywords:phospholipid;polymer monolayer;drug-eluting stent;biocompatible

[References]

Acharya G, Park K, Adv. Drug Deliv. Rev., 58, 387 (2006)
Kastrati A, et al., Am. J. Cardiol., 87, 34 (2001)
Linde J, Strauss BH, Expert Opin. Emerg. Drugs, 6, 281 (2001)
Rajagopal V, Rockson SG, Am. J. Med., 115, 547 (2003)
Kim TG, Lee H, Jang Y, Park TG, Biomacromolecules, 10(6), 1532 (2009)
Lee YK, et al., Biomaterials, 28, 1523 (2007)
Meng S, et al., Biomaterials, 30, 2276 (2009)
Pilgrim T, Windecker S, Minerva Cardioangiol, 57, 611 (2009)
Presbitero P, Asioli M, Minerva Cardioangiol, 50, 431 (2002)
Sindermann JR, et al., Carbohydr. Res., 63, 22 (2004)
Wang X, et al., Biomaterials, 29, 894 (2008)
Chapman D, Biochem. Soc. Trans., 21, 258 (1993)
Hayward JA, Chapman D, Biomaterials, 5, 135 (1984)
Korematsu A, et al., Biomaterials, 23, 263 (2002)
Mehilli J, et al., J. Am. Coll. Cardiol., 53, 1760 (2009)
Radeleff B, et al., Cardiovasc. Intervent Radiol., 31, 971 (2008)
Aleksiadi ER, Shaburishvili T, Georgian Med News, 10 (2007)
Guo Q, Knight PT, Mather PT, J. Control. Release, 137, 224 (2009)
Kim HK, Kim K, Byun Y, Biomaterials, 26, 3435 (2005)
Kim K, Kim C, Byun Y, J. Biomed. Mater. Res., 52, 836 (2000)
Kim K, Kim C, Byun Y, J. Biomech. Eng. -Trans. ASME, 14, 887 (2003)
Kim K, Kim C, Byun Y, Biomaterials, 25, 33 (2004)
Lee SR, et al., Circ. J., 72, 392 (2008)
Hasegawa T, Iwasaki Y, Ishihara K, Biomaterials, 22, 243 (2001)
Iwasaki Y, et al., Biomaterials, 23, 3897 (2002)
Hayward JA, Chapman D, Biomaterials, 5, 135 (1984)
Lao LL, Venkatraman SS, J. Control. Release, 130, 9 (2008)

[Related Articles]

[1] Acharya G, Park K, Adv. Drug Deliv. Rev., 58, 387 (2006)

[2] Kastrati A, et al., Am. J. Cardiol., 87, 34 (2001)

[3] Linde J, Strauss BH, Expert Opin. Emerg. Drugs, 6, 281 (2001)

[4] Rajagopal V, Rockson SG, Am. J. Med., 115, 547 (2003)

[5] Kim TG, Lee H, Jang Y, Park TG, Biomacromolecules, 10(6), 1532 (2009)

[6] Lee YK, et al., Biomaterials, 28, 1523 (2007)

[7] Meng S, et al., Biomaterials, 30, 2276 (2009)

[8] Pilgrim T, Windecker S, Minerva Cardioangiol, 57, 611 (2009)

[9] Presbitero P, Asioli M, Minerva Cardioangiol, 50, 431 (2002)

[10] Sindermann JR, et al., Carbohydr. Res., 63, 22 (2004)

[11] Wang X, et al., Biomaterials, 29, 894 (2008)

[12] Chapman D, Biochem. Soc. Trans., 21, 258 (1993)

[13] Hayward JA, Chapman D, Biomaterials, 5, 135 (1984)

[14] Korematsu A, et al., Biomaterials, 23, 263 (2002)

[15] Mehilli J, et al., J. Am. Coll. Cardiol., 53, 1760 (2009)

[16] Radeleff B, et al., Cardiovasc. Intervent Radiol., 31, 971 (2008)

[17] Aleksiadi ER, Shaburishvili T, Georgian Med News, 10 (2007)

[18] Guo Q, Knight PT, Mather PT, J. Control. Release, 137, 224 (2009)

[19] Kim HK, Kim K, Byun Y, Biomaterials, 26, 3435 (2005)

[20] Kim K, Kim C, Byun Y, J. Biomed. Mater. Res., 52, 836 (2000)

[21] Kim K, Kim C, Byun Y, J. Biomech. Eng. -Trans. ASME, 14, 887 (2003)

[22] Kim K, Kim C, Byun Y, Biomaterials, 25, 33 (2004)

[23] Lee SR, et al., Circ. J., 72, 392 (2008)

[24] Hasegawa T, Iwasaki Y, Ishihara K, Biomaterials, 22, 243 (2001)

[25] Iwasaki Y, et al., Biomaterials, 23, 3897 (2002)

[26] Hayward JA, Chapman D, Biomaterials, 5, 135 (1984)

[27] Lao LL, Venkatraman SS, J. Control. Release, 130, 9 (2008)