Protein Expression and Purification, Vol.72, No.2, 217-222, 2010
Zebrafish 10-formyltetrahydrofolate dehydrogenase is similar to its mammalian isozymes for its structural and catalytic properties
10-Formyltetrahydrofolate dehydrogenase from zebrafish has been cloned and expressed in both Escherichia coli and yeast. In addition, the N-terminal and C-terminal domains have also been cloned and expressed. Each expressed protein was purified to homogeneity and structural and kinetic properties determined. These studies show that the zebrafish enzyme is structurally and catalytically very similar to the enzymes from mammalian sources, suggesting that zebrafish can be used to study the in vivo function of 10-formyltetrahydrofolate dehydrogenase. (C) 2010 Elsevier Inc. All rights reserved.
Keywords:10-Formyltetrahydrofolate dehydrogenase;Folate;Folic acid;Folate-mediated one-carbon metabolism;Zebrafish;Enzyme