A batch recovery of riboflavin via foam separation from industrial simulative wastewater was studied using a cationic surfactant, cetyltrimethyl ammonium bromide (CTAB). The experimental parameters examined were the surfactant concentration, air flow rate, pH, and foam height. Under optimal operating conditions obtained through an orthogonal experiment, the maximum enrichment ratio of 48.7 was achieved for riboflavin along with 99.3% removal efficiency. The optimal operating conditions had the concentration of CTAB at 0.3 g/L, air flow rate at 400 ml/min, foam height at 90 cm, and pH at 12. Therefore foam separation proved to be an effective method to recover the riboflavin in terms of the good enrichment and removal efficiency.