Polyethersulfone-Activated carbon (PES-AC) hybrid particles are prepared using a liquid-liquid phase separation technique. The PES-AC hybrid particles are then used for the removal of phenobarbital (PB) from its aqueous solutions. The adsorption ability of the particles increased significantly when activated carbon was embedded into the particles, and with the increase of the activated carbon content in the hybrid particles, the PB adsorbed amount increased. Also when the PB concentration increased, the adsorbed amount increased. The adsorption of PB to the hybrid particles belongs to the Freundlich adsorption. To more effectively remove PB, PES-AC particle columns were prepared; and the particle column showed more high removal efficiency for PB. The results suggested that the PES -AC hybrid particles have a potential to be used for the removal of PB, and may be used in blood purification.