Biodegradation of monoethanolamine in aerobic and anoxic conditions

Dong-Jin Kim; Yeseul Lim; Daechul Cho; In Hyung Rhee

Korean Journal of Chemical Engineering, Vol.27, No.5, 1521-1526, September, 2010

DOI10.1007/s11814-010-0285-5 Export Citation

Biodegradation of monoethanolamine in aerobic and anoxic conditions

Dong-Jin Kim^†, Yeseul Lim, Daechul Cho¹, and In Hyung Rhee¹

Department of Environmental Sciences & Biotechnology, Hallym University, Gangwon 200-702, Korea
¹Department of Energy & Environmental Engineering, Soonchunhyang University, Chungnam 336-745, Korea

E-mail:

Monoethanolamine (MEA) is widely used in many industries and its proper treatment is important for protecting the water environment. As MEA contains an amine group, nitrogen removal by nitrification/denitrification as well as biodegradation of MEA is necessary for wastewater treatment. In this study the effects of adaptation and inhibition of MEA on biological degradation, and the removal of amine were investigated in a laboratory scale sequencing batch reactor (SBR). In addition, the denitrification characteristics of nitrate, and nitrite with MEA as the electron donor, were compared to the other electron donor (acetate). In the aerobic SBR, the removal efficiency of 9,000 mg/L MEA reached 92% at the hydraulic retention time (HRT) of 10.5 days. Ammonium hydrolyzed from the MEA was nitrified after 8 weeks from the start-up showing that adaptation time is needed for nitrification. Non-linear curve fitting of the specific MEA biodegradation gave the maximum specific activity (Vmax), the half saturation constant (Ks), and the inhibition constant (Ki) of 2.81 g/(g VSS·d), 102.1 mg/L, and 1149.6 mg/L, respectively. Batch denitrification showed that MEA is a competitive electron donor to acetate.

Keywords:Denitrification;Monoethanolamine;Nitrification;Sequencing Batch Reactor (SBR);Substrate Inhibition

[References]

Gallagher JR, Sorenson JA, Philbrick SS, Knutson RZ, Chollak D, Biol. Treat. Wastewater., 5, 269 (1995)
Hwang BJ, Park SW, Park DW, Oh KJ, Kim SS, Korean J. Chem. Eng., 26(3), 775 (2009)
Park SJ, Shin HY, Min BM, Cho A, Lee JS, Korean J. Chem. Eng., 26(1), 189 (2009)
Kim CJ, Sartori G, Int. J. Chem. Kinetics., 16, 1257 (1984)
Dawodu OF, Meisen A, Ind. Eng. Chem. Res., 33(3), 480 (1994)
Chen TK, Ni CH, Chen JN, J. Environ. Sci. Health Part A., 38, 2157 (2003)
Bakalova S, Mincheva V, Doycheva A, Groudeva V, Dimkov R, Biotechnol. Biotechnol. EQ., 22, 716 (2003)
Ndegwa AW, Wong RCK, Chu A, Bentely LR, Lunn SRD, J. Environ. Eng. Sci., 3, 137 (2004)
Hawthorne SB, Kubatova A, Gallagher JR, Sorensen JA, Miller DJ, Environ. Sci. Technol., 39, 3639 (2005)
Hyun JT, Rhee IH, Kwon SH, Kim DJ, Cho DC, Kor.J. Biotechnol. Bioeng., 22, 157 (2007)
Knapp JS, Jenkey ND, Townsley CC, Biodegradation., 7, 183 (1996)
Lai B, Shieh W, Water Res., 30, 2530 (1996)
Lei CN, Whang LM, Lin HL, Water Sci. Tech., 58, 1001 (2008)
APHA/AWWA/WEF, Standard methods for the examination of water and wastewater, 21st Ed., Washington DC (2005)
Hill GA, Robinson CW, Biotech. Bioeng., 17, 1599 (1975)
Water Environment Federation, Biological and chemical systems for nutrient removal, WEF, Alexandria (1998)
Sandin M, Allenmark S, Edebo L, FEMS Microbiol. Lett., 91, 147 (1992)
Verscheren K, Handbook of environmental data on organic chemicals, 4th Ed., Wiley, New York (2001)
Gernaey K, Verschuere L, Luyten L, Verstraete W, Water Environ.Res., 69, 1163 (1997)
Mrklas O, Chu A, Lunn S, Bentley LR, Water Air Soil Poll., 159, 249 (2004)
Goreau TJ, Kaplan WA, Wofsy SC, McElroy MB, Valois FW, Watson SW, Appl. Environ. Microbiol., 40, 526 (1980)
Anthonisen AC, Loehr RC, Prakasam TBS, Srinath EG, J. Wat. Poll. Cont. Fed., 48, 835 (1976)
Kaplan BH, Stadtman ER, J. Biol. Chem., 243, 1787 (1968)
Pochana K, Keller J, Water Sci. Technol., 39, 61 (1999)
Rittmann BE, McCarty PL, Environmental biotechnology: Principles and applications, McGraw-Hill, New York (2001)
McCarty PL, Biotechnol. Bioeng., 97(2), 377 (2007)

[Related Articles]

[1] Gallagher JR, Sorenson JA, Philbrick SS, Knutson RZ, Chollak D, Biol. Treat. Wastewater., 5, 269 (1995)

[2] Hwang BJ, Park SW, Park DW, Oh KJ, Kim SS, Korean J. Chem. Eng., 26(3), 775 (2009)

[3] Park SJ, Shin HY, Min BM, Cho A, Lee JS, Korean J. Chem. Eng., 26(1), 189 (2009)

[4] Kim CJ, Sartori G, Int. J. Chem. Kinetics., 16, 1257 (1984)

[5] Dawodu OF, Meisen A, Ind. Eng. Chem. Res., 33(3), 480 (1994)

[6] Chen TK, Ni CH, Chen JN, J. Environ. Sci. Health Part A., 38, 2157 (2003)

[7] Bakalova S, Mincheva V, Doycheva A, Groudeva V, Dimkov R, Biotechnol. Biotechnol. EQ., 22, 716 (2003)

[8] Ndegwa AW, Wong RCK, Chu A, Bentely LR, Lunn SRD, J. Environ. Eng. Sci., 3, 137 (2004)

[9] Hawthorne SB, Kubatova A, Gallagher JR, Sorensen JA, Miller DJ, Environ. Sci. Technol., 39, 3639 (2005)

[10] Hyun JT, Rhee IH, Kwon SH, Kim DJ, Cho DC, Kor.J. Biotechnol. Bioeng., 22, 157 (2007)

[11] Knapp JS, Jenkey ND, Townsley CC, Biodegradation., 7, 183 (1996)

[12] Lai B, Shieh W, Water Res., 30, 2530 (1996)

[13] Lei CN, Whang LM, Lin HL, Water Sci. Tech., 58, 1001 (2008)

[14] APHA/AWWA/WEF, Standard methods for the examination of water and wastewater, 21st Ed., Washington DC (2005)

[15] Hill GA, Robinson CW, Biotech. Bioeng., 17, 1599 (1975)

[16] Water Environment Federation, Biological and chemical systems for nutrient removal, WEF, Alexandria (1998)

[17] Sandin M, Allenmark S, Edebo L, FEMS Microbiol. Lett., 91, 147 (1992)

[18] Verscheren K, Handbook of environmental data on organic chemicals, 4th Ed., Wiley, New York (2001)

[19] Gernaey K, Verschuere L, Luyten L, Verstraete W, Water Environ.Res., 69, 1163 (1997)

[20] Mrklas O, Chu A, Lunn S, Bentley LR, Water Air Soil Poll., 159, 249 (2004)

[21] Goreau TJ, Kaplan WA, Wofsy SC, McElroy MB, Valois FW, Watson SW, Appl. Environ. Microbiol., 40, 526 (1980)

[22] Anthonisen AC, Loehr RC, Prakasam TBS, Srinath EG, J. Wat. Poll. Cont. Fed., 48, 835 (1976)

[23] Kaplan BH, Stadtman ER, J. Biol. Chem., 243, 1787 (1968)

[24] Pochana K, Keller J, Water Sci. Technol., 39, 61 (1999)

[25] Rittmann BE, McCarty PL, Environmental biotechnology: Principles and applications, McGraw-Hill, New York (2001)

[26] McCarty PL, Biotechnol. Bioeng., 97(2), 377 (2007)