Tunable multicolor organic patterns based on two dye molecules are demonstrated. The left-hand fluorescence microscopy image shows the emission for 32 nm DtCDQA on narrow Au lines and 1 nm on the SiO2 substrate. Subsequent deposition of the second dye, NPB, can create tunable multicolor patterns by controlling the emission from NPB and the monomer and aggregates of DtCDQA. Depositing 10 nm NPB gives orange on green (upper right), and 100 nm NPB, green on blue (lower right).