A plug flow reactor (PFR) which permits the growth of bacteria in a microwave environment is described. The apparatus is used to analyse the effect of microwave radiation at the frequency of 2.45 GHz and at 37 degrees C on the growth rate of three bacterial species: Bacillus clausii, Pseudomonas aeruginosa, and Staphylococcus aureus. The growth constant is determined for reacting mixtures irradiated with microwaves of different powers ranging from 0 to 400 mW. Analyses show that (a) the apparatus is able to perform the experimental runs maintaining an aseptic environment; (h) under the microwave power levels examined in the present study, no effects are detected for the Bacillus clausii and Pseudomonas aeruginosa species, while for the Staphylococcus aureus species, a few small effects are found (a minimum value of the growth constant at 200 mW and an increase of the constant between 200 and 400 mW). (C) 2009 American Institute of Chemical Engineers AIChE J, 56: 1270-1278, 2010