EFFECTS AND OPTIMIZATION OF SELECTION PRESSURE IN ENZYME PRODUCTION USING RECOMBINANT E. coli

LEE JH; YOO YJ; PAR KH

Korean Journal of Chemical Engineering, Vol.8, No.1, 39-43, January, 1991

Selection pressure is widely used in stabilizing the plasmid of the recombinant microorganism. The effects of selection pressure on enzyme production were investigated using recombinant E. coli harboring plasmid pTA322. Optimum ampicillin concentration which maximizes the enzyme production was 150㎍/㎖ in flask culture and 50㎍/㎖ in the culture using 2-1 fermentor. Optimum concentration of selection pressure in a stirred fermentor was different from that in a flask due to different agitation effects. It was found from mathematical modelling that as the concentration of ampicillin increased, the plasmid-harboring cells with low plasmid copy number were damaged severely, while the specific production rate increased due to the high plasmid copy number in the microorganism.

[References]

Layman PL, Chem. Eng. News, 11 (1989)
Underkofler LA, Bioeng. Food Process., 62, 11 (1969)
Georgiou G, AIChE J., 34, 1233 (1988)
Karl E, Christine LH, Adv. Biochem. Eng. Biotechnol., 26, 143 (1983)
Zabriskie DW, Arcuri EJ, Enzyme Microb. Technol., 8, 706 (1986)
Lee SB, Bailey JE, Biotechnol. Bioeng., 26, 66 (1984)
Hjortso MA, Bailey JF, Biotechnol. Bioeng., 26, 528 (1984)
Siegel R, RyuDewey DY, Biotechnol. Bioeng., 27, 28 (1985)
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Dennis K, Srienc F, Bailey JE, Biotechnol. Bioeng., 27, 1490 (1985)
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Toda K, J. Chem. Technol. Biotechnol., 31, 775 (1981)
Imanaka T, Aiba S, Annu. N.Y. Acad. Sci., 369, 1 (1981)
Kim SH, RyuDewey DY, Biotechnol. Bioeng., 26, 497 (1984)
Koizumi JT, Aiba S, Biotechnol. Bioeng., 28, 311 (1986)

[Referenced By]

[1] Layman PL, Chem. Eng. News, 11 (1989)

[2] Underkofler LA, Bioeng. Food Process., 62, 11 (1969)

[3] Georgiou G, AIChE J., 34, 1233 (1988)

[4] Karl E, Christine LH, Adv. Biochem. Eng. Biotechnol., 26, 143 (1983)

[5] Zabriskie DW, Arcuri EJ, Enzyme Microb. Technol., 8, 706 (1986)

[6] Lee SB, Bailey JE, Biotechnol. Bioeng., 26, 66 (1984)

[7] Hjortso MA, Bailey JF, Biotechnol. Bioeng., 26, 528 (1984)

[8] Siegel R, RyuDewey DY, Biotechnol. Bioeng., 27, 28 (1985)

[9] Hopkins DJ, Betenbaugh MJ, Bhurjati P, Biotechnol. Bioeng., 29, 85 (1987)

[10] Pierce J, Guteridge S, Appl. Environ. Microbiol., 49, 1094 (1985)

[11] Dennis K, Srienc F, Bailey JE, Biotechnol. Bioeng., 27, 1490 (1985)

[12] Kim IC, Jang SY, Cha JH, Ko YH, Park KH, Rho HM, Korean J. Appl. Microbiol. Bioeng., 16, 369 (1988)

[13] Fuwa H, J. Biochem., 41, 583 (1954)

[14] Perry RH, "Perry's Chemical Engineers' Handbook," 3-75 and 3-249, McGraw-Hill, New York (1985)

[15] Toda K, J. Chem. Technol. Biotechnol., 31, 775 (1981)

[16] Imanaka T, Aiba S, Annu. N.Y. Acad. Sci., 369, 1 (1981)

[17] Kim SH, RyuDewey DY, Biotechnol. Bioeng., 26, 497 (1984)

[18] Koizumi JT, Aiba S, Biotechnol. Bioeng., 28, 311 (1986)