Porous silicon fabricated via Pt-assisted chemical etching of p-type Si (1 0 0) in 1: 1: 1 EtOH/HF/H2O2 solution possesses a longer durability in air and in aqueous media than anodized one, which is advantageous for biomedical applications. Its surface SiHx (x = 1 and 2) species can react with 10-undecylenic acid completely under microwave irradiation, and subsequent derivatizations of the end carboxylic acid result in affinity capture of proteins. We applied two approaches to produce protein microarrays: photolithography and spotting. The former provides a homogeneous microarray with a very low fluorescence background, while the latter presents an inhomogeneous microarray with a high noise background. (C) 2010 Elsevier B. V. All rights reserved.