In this study TiO2 nanotube arrays were fabricated by potentiostatic anodization of titanium sheet. The Xray diffraction (XRD) pattern and field emission scanning electron microscopy (FE-SEM) image indicated the TiO2 nanotube arrays were of pure anatase form and highly ordered. The properties of the photogenerated charges in the nanotube arrays were investigated by transient photovoltage (TPV) technique and surface photovoltage (SPV) technique based on lock-in amplifier with dc bias, in comparison with the commercial powder derived film. The separation processes of the photo-induced charges in the system of TiO2 nanotubes on Ti have been demonstrated to be correlated with the incident light intensity, surface trapping states, and the interfacial electric field between Ti and TiO2. The results also show that the highly ordered nanotube film could generate much stronger SPV responses under external electric field than the commercial powder derived film. (C) 2010 Elsevier B.V. All rights reserved.