Wetting properties of silica plates contacted with dipalmitoylphosphatidylcholine (DPPC) or DPPC/enzyme (phospholipase PLA(2)) in NaCl solution were determined by thin layer wicking and with a help of Washburn equation. The wicking experiments were performed both for bare plates and the silica plates precontacted overnight with the probe liquid saturated vapors the silica plates, as well as untreated and DPPC (or DPPC/enzyme) treated. Adsorption of DPPC on original silica plates increases a bit hydrophobic character of silica surface in such a way that hydrocarbon chains are directed outwards and the polar part towards the silica surface. However, after the enzyme action the products of DPPC hydrolysis by PLA(2) (palmitic acid and lysophosphatidylcholine) increase again hydrophilic character of silica surface (an increase in acid-base interactions, gamma(AB)(s)). The changes of silica surface wettability are evidently dependent on the time of enzyme contacting with DPPC in NaCl solution. Although, the changes of total surface free energy of silica after treatment with DPPC/enzyme solution are minor about 2-6 mJ/m(2), the changes of the electron-donor (gamma(-)(s)) and Lifshitz-van der Waals (gamma(LW)(s)) component of the surface free energy are noticeable. Despite, these results are somehow preliminary, it seems that thin layer wicking method is an interesting tool for investigation of the effect of adsorbed DPPC on hydrophobicity/hydrophilicity of silica surface and influence of enzyme PLA(2) action. (C) 2010 Elsevier B.V. All rights reserved.