Synthesis of Poly(L,L-lactic acid-co-lysine) and Its Cell Compatibility Evaluation as Coating Material on Metal

Li Jie Duan; Min Ji Kim; Jik Han Jung; Dong June Chung; Jiheung Kim

Macromolecular Research, Vol.18, No.8, 806-811, August, 2010

DOI10.1007/s13233-010-0809-1 Export Citation

Synthesis of Poly(L,L-lactic acid-co-lysine) and Its Cell Compatibility Evaluation as Coating Material on Metal

Li Jie Duan, Min Ji Kim, Jik Han Jung, Dong June Chung^†, and Jiheung Kim¹

Department of Polymer Science and Engineering, Sungkyunkwan University, Suwon, 440-746, Korea
¹Department of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea

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This study evaluated and compared the cell adhesion and spreading behavior on the surfaces of biodegradable poly(L,L-lactic acid) (PLA) and poly(L,L-lactic acid-co-lysine) (PLAL) films. The thermal stability and film surface properties were examined by differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), contact angle, and scanning electron microscopy (SEM) to assess the physicochemical characteristics affecting the cell adhesion behavior of materials, PLA and PLAL. Mouse fibroblast cells were cultured on the surface of stainless steel wafers coated with PLA and PLAL. SEM images showed most of the cells spreading slightly on the PLA coating film with a linkage of cellular pseudopods. The degree of cell spreading on the PLAL surface would be higher than that of PLA due to the side-chain effect of (+) charged amino groups easily inducing cell adhesion and spreading. Moreover, the cell viability was also evaluated on PLA and PLAL using a MTT assay, and no acute cytotoxicity was observed.

Keywords:poly(lactic acid-co-lysine);cell adhesion and spreading;cytotoxicity;polyelectrolyte interaction

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Kim JK, Lee JI, Lee DH, Macromol. Res., 16(4), 267 (2008)
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Rose SF, Lewis AL, Hanlon GW, Lloyd AW, Biomaterials, 25, 5125 (2004)

[Related Articles]

[1] Nair LS, Laurencin CT, Prog. Polym. Sci, 32, 762 (2007)

[2] Barrera DA, Zylstra E, Lansbury PT, Langer R, J. Am. Chem. Soc., 115, 11010 (1993)

[3] Deng C, Chen XS, Yu HJ, Sun J, Lu TC, Jing XB, Polymer, 48(1), 139 (2007)

[4] Yu H, Guo XJ, Qi XL, Liu PF, Shen XY, Duan YR, J. Mater. Sci. -Mater. Med., 19, 1275 (2008)

[5] Barrera DA, Zylstra E, Lansbury PT, Langer R, Macromolecules, 28(2), 425 (1995)

[6] Cook AD, Hrkach JS, Gao NN, Johnson IM, Pajvani UB, Cannizzaro SM, Langer R, J. Biomed. Mater. Res., 35, 513 (1997)

[7] Caponetti G, Hrkach JS, Kriwet B, Poh M, Lotan N, Colombo P, Langer R, J. Pharmaceut. Sci., 88, 136 (1999)

[8] Kidchob T, Kimura S, Imanishi Y, J. Control. Release, 54, 283 (1998)

[9] Kimura S, Kidchob T, Imanishi Y, Polym. Adv. Technol., 12, 85 (2001)

[10] Burt HM, Hunter WL, Adv. Drug Deliv. Rev., 58, 350 (2006)

[11] Shin YM, Lim KS, Jin JY, Jeong SI, Lee YM, Shin H, Kim KS, Macromol. Res., 17(12), 1039 (2009)

[12] Koster R, Vieluf D, Kiehn M, Sommerauer M, Kahler J, Baldus S, Meinertz T, Hamm CW, Lancet, 356, 1895 (2000)

[13] Mani G, Feldman MD, Patel D, Agrawal CM, Biomaterials, 28, 1689 (2007)

[14] Tanabe K, Regar E, Lee CH, Hoye A, van der Giessen WJ, Serruys PW, Curr. Pharm. Des., 10, 357 (2004)

[15] Venkatraman S, Boey F, Lao LL, Prog. Polym. Sci, 33, 853 (2008)

[16] Kim JK, Lee JI, Lee DH, Macromol. Res., 16(4), 267 (2008)

[17] Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR, J. Natl. Cancer. Inst., 82, 1107 (1990)

[18] Xu F, Cui FZ, Jiao YP, Meng QY, Wang XP, Cui XY, J. Mater. Sci. -Mater. Med., 20, 1331 (2009)

[19] Awual MR, Jyo A, Water Res., 43, 1229 (2009)

[20] Kim DG, Choi C, Jeong YI, Jang MK, Nah JW, Kang SK, Bang MS, Macromol. Res., 14(1), 66 (2006)

[21] Rose SF, Lewis AL, Hanlon GW, Lloyd AW, Biomaterials, 25, 5125 (2004)