Mesoporous nickel-alumina (Ni-A-NS) catalysts prepared by a non-ionic surfactant-templatingmethod were calcined at various temperatures for use in hydrogen production by steam reforming of liquefied natural gas (LNG). The effect of calcination temperature of nickel.alumina catalysts on their physicochemical properties and catalytic activity for steam reforming of LNG was investigated. Nickel oxide species were finely dispersed on the surface of Ni-A-NS catalysts through the formation of nickel aluminate phase. Reducibility, nickel surface area, and nickel dispersion of Ni-A-NS catalysts decreased with increasing calcination temperature. In the steam reforming of LNG, both LNG conversion and hydrogen composition in dry gas decreased with increasing calcination temperature of Ni-A-NS catalysts. Nickel surface area and reducibility of Ni-A-NS catalysts were well correlated with catalytic performance of the catalysts. Among the catalysts tested, Ni-A-NS700 (nickel.alumina catalyst calcined at 700 ℃) with the highest nickel surface area and the highest reducibility exhibited the best catalytic performance.