A new monoamine oxidase type C-like dehydratase gene (MaoC) supplying (R)-3-hydroxyacyl-CoA from the fatty acid oxidation pathway to polyhydroxyalkanoates (PHAs) biosynthetic pathways was identified from Phytophthora capsici. MaoC was over-expressed in Escherichia coli and the recombinant MaoC was purified. The purified tagged MaoC shows the enoyl-CoA hydratase activity of 58 U/mg towards crotonyl-CoA. MaoC may not fold properly above 40A degrees C which was revealed by circular dichroism analysis. Crystal of MaoC diffracts to 2.9 in space group P2(1)2(1)2(1), with unit-cell parameters a = 81.5 , b = 82.6 , c = 124.2 , alpha = beta = gamma = 90A degrees.