Turbulent flow inside a dished bottom baffled stirred tank reactor (STR) with a 45 degrees pitched blade impeller is studied numerically and experimentally. Three different impeller rotational speeds are studied corresponding to impeller Reynolds numbers of 44,000, 88,000 and 132,000, respectively. The numerical study is based on a large-eddy simulation (LES) technique with a fixed body-fitted curvilinear mesh. The moving impeller geometries are modeled using an immersed boundary method (IBM). The experiments consist of particle image velocimeter (PIV) measurements of the flow field. The instantaneous as well as the time-averaged flow field suggests the formation of trailing vortex structures which are associated with higher levels of turbulent kinetic energy relative to the remaining flow field. Instabilities occurring at a frequency lower than the frequency of impeller rotation are identified from the time signal of the velocity components. The role of these lower frequency macro-instabilities (MI) is explored by observing changes in the three-dimensional circulation pattern within the stirred tank. The growth and dissipation of trailing edge vortices are shown to be appreciably influenced by the macro-instability. A significant amount of kinetic energy (velocity fluctuations) is observed to be associated with the low frequency dynamics of the trailing edge vortices during an MI cycle. (C) 2010 Elsevier Ltd. All rights reserved.