Surface enhanced Raman scattering (SERS) spectra of Rhodamine 6G adsorbed on silver nanoparticles have been measured using an optical fiber from 22 to 85 degrees C. The fiber carries both the laser excitation and SERS signal, providing a convenient scheme for in-situ SERS measurement at high-temperature microscale environments. It is found that although SERS intensity generally decreases with increasing temperature, the signal is still significant and stable at high-temperature up to 85 degrees C. More importantly, the SERS signal is reversible with respect to the temperature change. These results are important for high-temperature SERS applications in chemical and biological detection. (C) 2010 Elsevier B.V. All rights reserved.