A triangular dehydrobenzo[12]annulene (DBA) molecule bridged between Au electrodes was investigated by scanning tunneling microscopy break junction technique. The conductance of the single DBA molecule was determined to be 2.5 x 10(-5) G(0) (G(0) = 2e(2)/h). Though the DBA has three anchor units which can bind to metal electrodes, the conductance measurement revealed that two of three anchor units per molecule are most likely to bind to the Au electrodes to form a single molecular junction.