Electrospinning has been regarded as a convenient method of manufacturing polymer-based multi-functional and high performance nanofibres that can significantly contribute to the world of advanced materials. The primary requirement of the process is to obtain nanofibres in continuous form with fine diameters and minimum variations. Secondly, the fibre network has to have minimum area occupied by beads to enhance the network's porosity. These two important characteristics, when achieved, render the nanofibre mats acceptable for many membrane type applications. The relationship between processing parameters and microstructures of nanofibrils is still not well understood. The goals of this study are to obtain a set of robust manufacturing parameters that would reduce the variation in quality while electrospinning non-woven mats of nanofibres from poly(L-lactic) acid (PLLA). The study involves sixteen sets of parametric combinations and the scanning electron microscopy of the produced mats. The desirable combination for producing acceptable networks appears to be low concentration of polymer solution, low feed rate, comparatively high applied voltage and a large distance (within the studied range) between the collector and the needle. However, a low concentration of polymer solution may result in some bead formation if other factors are not changed accordingly. (C) Koninklijke Brill NV, Leiden, 2010