A MATHEMATICAL MODEL FOR THE REGULATION OF TRYPTOPHAH PROMOTER

Park TH

Korean Journal of Chemical Engineering, Vol.11, No.4, 282-289, October, 1994

Tryptophan promoter is widely used in the industrial fermentation for the overproduction of recombinant proteins. It can be regulated by the concentrations of tryptophan and 3β-indoles-crylic acid(IAA). A mathematical model is proposed for the regulation of the promoter by the manipulation of tryptophan and IAA concentrations. The transcription initiation frequency increases with IAA concentration and reaches a plateau. The frequency decreases drastically even with the addition of a small amount of tryptophan. These behaviors agree behaviors agree with the previous experimental investigation.

[References]

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Emtage JS, Tacon WC, Catlin GH, Jenkins B, Porter AG, Carey NH, Nature, 283, 171 (1980)
Leung DW, Capon DJ, Goeddel DV, Biotechnol., 2, 458 (1984)
Rosenberg SA, Grimm EA, McGrogan M, Doyle M, Kawasaki E, Koths K, Mark DF, Science, 223, 1412 (1984)
Park TH, Seo JH, Lim HC, Biotechnol. Bioeng., 34, 1167 (1989)
Yoon SK, Kang WK, Park TH, Biotechnol. Bioeng., 43(10), 995 (1994)
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Imamoto F, Proc. Natl. Acad. Sci. U.S.A., 60, 305 (1968)
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Jackson EN, Yanofsky C, J. Mol. Biol., 69, 307 (1972)
Yanofsky C, Kelley RL, Horn V, J. Bacteriol., 158, 1018 (1984)
Park TH, Seo JH, Lim HC, Biotechnol. Lett., 11, 87 (1989)
Shinha S, Biotechnol. Bioeng., 31, 117 (1988)
Koh BT, Yap MGS, Biotechnol. Bioeng., 41, 707 (1993)
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Zalkin H, "Multifunctional Enzymes," eds. Bisswanger, H. and Schmincke-Ott, Ed., 1213, John Wiley and Sons, Inc., New York (1980)
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[Referenced By]

[1] Rosenberg M, Court D, Ann. Rev. Genet., 13, 319 (1979)

[2] Emtage JS, Tacon WC, Catlin GH, Jenkins B, Porter AG, Carey NH, Nature, 283, 171 (1980)

[3] Leung DW, Capon DJ, Goeddel DV, Biotechnol., 2, 458 (1984)

[4] Rosenberg SA, Grimm EA, McGrogan M, Doyle M, Kawasaki E, Koths K, Mark DF, Science, 223, 1412 (1984)

[5] Park TH, Seo JH, Lim HC, Biotechnol. Bioeng., 34, 1167 (1989)

[6] Yoon SK, Kang WK, Park TH, Biotechnol. Bioeng., 43(10), 995 (1994)

[7] Kawai S, Mizutani S, iijima S, Kobayashi T, J. Ferment. Technol., 64, 503 (1986)

[8] Baker RF, Yanofsky C, Proc. Natl. Acad. Sci. U.S.A., 60, 313 (1968)

[9] Imamoto F, Proc. Natl. Acad. Sci. U.S.A., 60, 305 (1968)

[10] Morse DE, Mosteller RD, Yanofsky C, Cold Spring Harbor Symp. Quant. Biol., 34, 725 (1969)

[11] Baker R, Yanofsky C, Cold Spring Harbor Symp. Quant. Biol., 35, 467 (1970)

[12] Baker R, Yanofsky C, J. Mol. Biol., 69, 89 (1972)

[13] Bertrand K, Squires C, Yanofsky C, J. Mol. Biol., 103, 319 (1976)

[14] Jackson EN, Yanofsky C, J. Mol. Biol., 69, 307 (1972)

[15] Yanofsky C, Kelley RL, Horn V, J. Bacteriol., 158, 1018 (1984)

[16] Park TH, Seo JH, Lim HC, Biotechnol. Lett., 11, 87 (1989)

[17] Shinha S, Biotechnol. Bioeng., 31, 117 (1988)

[18] Koh BT, Yap MGS, Biotechnol. Bioeng., 41, 707 (1993)

[19] Joachimiak AR, Kelley L, Gunsalus RP, Yanofsky C, Sigler P, Proc. Natl. Acad. Sci. U.S.A., 80, 668 (1983)

[20] Joachmimiak AR, Schevitz RW, Kelley L, Yanofsky C, Sigler P, J. Biol. Chem., 258, 12641 (1983)

[21] Schevitz RW, Otwinowski Z, Joachimiak A, Lawson CL, Sigler PB, Nature, 317, 786 (1985)

[22] Arvidson DN, Bruce C, Gunsalus RP, J. Biol. Chem., 261, 238 (1986)

[23] Joseph DR, Muench KH, J. Biol. Chem., 246, 7602 (1971)

[24] Joseph DR, Muench KH, J. Biol. Chem., 246, 7610 (1971)

[25] Pabst MJ, Kuhn JC, Somerville RL, J. Biol. Chem., 248, 910 (1973)

[26] Zalkin H, "Multifunctional Enzymes," eds. Bisswanger, H. and Schmincke-Ott, Ed., 1213, John Wiley and Sons, Inc., New York (1980)

[27] Das A, Drawfold IP, Yanofsky C, J. Biol. Chem., 257, 8795 (1982)

[28] Haydock PV, Somerville RL, Biochem. Biophys. Res. Commun., 119, 926 (1984)

[29] Rose JK, Squires CL, Yanofsky C, Yang HL, Zubay G, Nature New Biol., 245, 133 (1973)

[30] ZubayG, Morse DE, Schrenk WJ, Miller JH, Proc. Natl. Acad. Sci. U.S.A., 69, 1100 (1972)

[31] Squires CL, Lee F, Yanofsky C, J. Mol. Biol., 92, 93 (1975)

[32] Kelley RL, Yanofsky C, Proc. Natl. Acad. Sci. U.S.A., 79, 3120 (1982)

[33] Yanofsky C, Crawford IP, "E. Coli and Salmonella Typhimurium Vol. 2," eds. Neidthard, F.C., Ingraham, J.L., Low, K.B., Magasanik, B. and Schaechter, M., 1453, American Society for Microbiology, Washington D.C. (1987)