항 아토피 화장품이 아토피 동물모델 Hairless Mice에 미치는 영향

권택관; 임건빈; 김진철; Taek Kwan Kwon; Kun Bin Lim; Jin-Chul Kim

Applied Chemistry for Engineering, Vol.22, No.1, 91-97, February, 2011

Export Citation

항 아토피 화장품이 아토피 동물모델 Hairless Mice에 미치는 영향

The Effect of Anti-atopic Cosmetic in Hairless Mice

권택관, 임건빈¹, 김진철 ^†

강원대학교 바이오산업공학부 생물소재공학전공
¹화인코주식회사

Taek Kwan Kwon, Kun Bin Lim¹, and Jin-Chul Kim^†

Division of Biotechnology & Bioengineering and Institute of Bioscience and Biotechnology, Kangwon National University, Kangwon-do 200-701, Korea
¹FineCo Ltd, Bio Venture Plaza, Kangwon-do 200-160, Korea

E-mail:

초록

이 연구는 아토피 피부염이 유발된 hairless mice에 항 아토피 제품 처리를 통해 아토피 증상 개선 효과에 대해 알아본 실험이다. 항 아토피 물질인 ceramide와 astaxanthin을 melt-homogenization 방법을 이용해 고형 지질 나노입자를 제조하고 입자 주위에 silk fibroin을 코팅시켰다. 동시에 고형 지질 나노입자를 함유하는 항 아토피 로션(lotion)과 크림(cream)을 만들어 타 사의 항 아토피 제품과 비교하였다. 아토피 피부염이 유발된 마우스의 외형적인 모습 관찰을 통해 ceramide와 silk fibroin을 함유한 항 아토피 제품인 FL과 FC는 아토피 피부염으로 손상된 피부 장벽을 재생 시키는 것을 확인하였고, 아토피 피부염이 유발된 피부 두께 역시 감소됨을 알 수 있었다. 면역학적 평가를 통해 silk fibroin과 astaxanthin 이 Th2 cytokine인 IgE와 IL-4의 발현을 억제하고 IFN-γ의 발현을 증가시켜 Th cell 사이의 균형을 이루게 하는 것이 밝혀졌다. 또한, 비장수치를 통해 T 림파구의 따른 IgE, IL-4 발현 억제능, 그리고 IFN-γ 발현을 확인하였다.

The efficacies of anti-atopic preparations were investigated in hairless mice suffering from 2,4-dinitro-chlorobenzene(DNCB)-induced atopic dermatitis-like lesion. Solid lipid nanoparticle (SLN) containing ceramide and astaxanthin was prepared by a melt-homogenization method using Aminsoft-CT 12 (Cocoyl glutamate) as an emulsifier. And then, the SLNs were coated with silk fibroin (SF) by taking advantage of an electrostatic interaction between the surface of SLNs and SF. SLNs were included in lotion (FL) and cream (FC) types of preparations. Anti-atopic efficacies of the preparations were investigated in terms of appearance of skin surface, spleen index, serum IgE level, and serum cytokine level. SLN-containing preparations suppressed IgE production and IL-4 expression, but promoted IFN-γexpression.

Keywords:ceramide;astaxanthin;atopic dermatitis (AD);immunoglobulin E;cytokine

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Cho SM, Lee HY, Kim JC, J. Am. Oil. Chem., 84, 859 (2007)
Matsumoto K, Mizukoshi K, Oyobikawa M, Ohshima H, Sakai Y, Tagami H, Skin Res. Technol., 11, 209 (2005)
Kim DW, Park JY, Na GY, Lee SJ, Lee WJ, Int.J. Dermatol., 45, 698 (2006)
Loden M, Olsson H, Axell T, Linde YW, Br. J. Dermatol., 126, 137 (2006)
Haug S, Roll A, Schmid-Grendelmeier P, Johansen P, Wuthrich B, Kundig TM, Senti G, Curr. Probl. Dermatol., 33, 144 (2006)
Agner T, Contact Dermatitis., 25, 108 (2006)
Jyonouchi H, Sun S, Tomita Y, Gross MD, J. Nutr., 125, 2483 (1995)
Walker C, Kagi MK, Ingold P, Braun P, Blaser K, Bruijnzeel-Koomen CAFM, Wuthrich B, Exp. Allergy., 23, 145 (2006)

[Related Articles]

[1] Cooper KD, J. Invest. Dermatol., 102, 128 (1994)

[2] Soter NA, Allergy., 44, 16 (1989)

[3] Uehara M, Izukura R, Sawai T, Clin. Exp. Dermatol., 15, 264 (1990)

[4] Donald YM, Leung M, J. Allergy Clin. Immunol., 105, 860 (2000)

[5] Qi XF, Kim DH, Yoon YS, Li JH, Jin D, Deung YK, Lee KJ, J. Ethnopharmacol., 123, 195 (2009)

[6] Poulsen LK, Reimert CM, Bindslev-Jensen C, Hansen MB, Bendtzen K, Int. Arch. Allergy Immunol., 106, 55 (1995)

[7] Ahn JY, Im LR, Kim JH, Park JH, Kim DK, Lee YM, Kor. J. Pharmacogn., 40, 218 (2009)

[8] Hannun YA, Obeid LM, Trends Biochem. Sci., 20, 73 (1995)

[9] Chamlin SL, Kao J, Frieden IJ, Sheu MY, Fowler AJ, Fluhr JW, Williams ML, Elias PM, J. Am. Acad. Dermatol., 47, 198 (2002)

[10] Linan-Cabello MA, Paniagua-Michel J, Hopkins PM, Aquac.Nutr., 8, 299 (2002)

[11] Naguib YMA, J. Agric. Food Chem., 48, 1150 (2000)

[12] Sakai S, Sugawara T, Matsubara K, Hirata T, J. Biol.Chem., 284, 28172 (2009)

[13] Kang MJ, Eum JY, Jeong MS, Choi SE, Park SH, Cho HI, Cho CS, Seo SJ, Lee MW, Choi YW, Biol.Pharm. Bull., 33, 10 (2010)

[14] Cho SM, Lee HY, Kim JC, J. Am. Oil. Chem., 84, 859 (2007)

[15] Matsumoto K, Mizukoshi K, Oyobikawa M, Ohshima H, Sakai Y, Tagami H, Skin Res. Technol., 11, 209 (2005)

[16] Kim DW, Park JY, Na GY, Lee SJ, Lee WJ, Int.J. Dermatol., 45, 698 (2006)

[17] Loden M, Olsson H, Axell T, Linde YW, Br. J. Dermatol., 126, 137 (2006)

[18] Haug S, Roll A, Schmid-Grendelmeier P, Johansen P, Wuthrich B, Kundig TM, Senti G, Curr. Probl. Dermatol., 33, 144 (2006)

[19] Agner T, Contact Dermatitis., 25, 108 (2006)

[20] Jyonouchi H, Sun S, Tomita Y, Gross MD, J. Nutr., 125, 2483 (1995)

[21] Walker C, Kagi MK, Ingold P, Braun P, Blaser K, Bruijnzeel-Koomen CAFM, Wuthrich B, Exp. Allergy., 23, 145 (2006)