클로로프렌 고무로 결합된 동물조직 고정 탄소반죽 전극의 전기화학적 특성

유근배; Keun-Bae Rhyu

Applied Chemistry for Engineering, Vol.22, No.1, 114-118, February, 2011

Export Citation

클로로프렌 고무로 결합된 동물조직 고정 탄소반죽 전극의 전기화학적 특성

Electrochemical Characterization of Animal Tissue-Immobilized Carbon Paste Biosensor Bound with Chloroprene Rubber

유근배^†

청주대학교 자연과학부 응용화학과

Keun-Bae Rhyu^†

Department of Applied Chemistry, Cheongju University, Cheongju 360-764, Korea

E-mail:

초록

미네랄 오일을 결합재로 사용하는 탄소반죽전극은 효소의 특성 연구 분야에서 효과적으로 활용되고 있으나 전극이 기계적 경도가 없으므로 실용화의 가능성은 없다. 본 실험실에서는 톨루엔에 녹인 고무 용액이 탄소가루의 결합재로 사용될 때 탄소반죽의 기계적 물성이 얻어지는 것을 확인하였다. 클로로프렌 고무 용액을 이용하여 만든 전극이 정량적인 전기화학적 행동을 보이는지 확인하기 위하여 여러 가지 속도론적 파라메터 즉 대칭인자(α, 0.28), 교환전류밀도 (i0, 4.06 μA/cm2), 이중층의 축전용량(Cd, 2.11 × 10^(-3)F), Michaelis 상수(KM, 2.45 × 10^(-3)M), 시간상수(τB, 0.077 sec) 등을 구하였다. 이 실험적 사실들은 클로로프렌 고무가 탄소반죽전극의 실용화에 전망 좋은 결합재임을 입증하였다.

Even though the carbon paste electrode bound with mineral oil is useful for research about the characteristics of enzymes, it remains far from practical uses because the lack of mechanical hardness limits its practical use. When the rubber liquefied in toluene was used as a binder of carbon powder in lab, it is confirmed that the mechanical robustness of the electrode is guaranteed. In order to confirm whether it shows quantitative electrochemical behaviors or not, its kinetic parameters, e.g. the symmetry factor (α= 0.28), the exchange current density (i0 = 4.06 μA/cm2), the capacity of the double layer (Cd =2.11 × 10^(-3)F), the Michaelis constant (KM = 2.45 × 10^(-3)M), and the time constant (τB = 0.077 sec) were investigated. Our experimental observations prove that the chloroprene rubber is a promising binder for the practical use of a carbon paste electrode.

Keywords:carbon paste electrode;hydrogen peroxide;peroxidase;chloroprene rubber

[References]

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[1] Cheng TJ, Lin TM, Chang HC, Anal. Chim. Acta., 462, 261 (2002)

[2] Roy JJ, Abraham TE, Abhijith KS, Kumar PVS, Thakur MS, Biosens. Bioelectron., 21, 206 (2005)

[3] Yang YF, Mu SL, Biosens. & Bioelectron., 21, 74 (2005)

[4] Li YC, Bu WF, Wu LX, Sun CQ, Sens. Acturators B., 107, 921 (2005)

[5] Chen X, Zhang JZ, Wang BQ. Cheng GJ, Dong SJ, Anal. Chim. Acta., 434, 255 (2001)

[6] Miquel AS, Arben M, Salvador A, Sens. Acturators B., 69, 153 (2000)

[7] Tingry S, Innocent C, Touil S, Deratani A, Seta P, Mater.Sci. Eng. C., 26, 222 (2006)

[8] Wang J, Mo JW, Li SF, Porter J, Anal. Chim. Acta., 441, 813 (2001)

[9] Yoon KJ, Kim KJ, Kwon HS, J. Kor. Chem. Soc., 43, 271 (1999)

[10] Yoon KJ, Anal. Sci. Tech., 16, 504 (2003)

[11] Yoon KJ, J. Kor. Chem. Soc., 48, 654 (2004)

[12] Yoon KJ, Bull. Kor. Chem. Soc., 25, 997 (2004)

[13] Yoon KJ, Bull. Kor. Chem. Soc., 29, 2264 (2008)

[14] Lee BG, Rhyu KB, Yoon KJ, Bull. Kor. Chem. Soc., 30, 2457 (2009)

[15] Lee BG, Rhyu KB, Yoon KJ, J. Ind. Eng. Chem., 16(3), 340 (2010)

[16] Brydson, Rubbery Materials and Their Compounds, Elsvier Applied Science, London and New York, 206 (1988)

[17] Pandey PC, Upadhyay S, Upadhyay B, Anal. Biochem., 252, 136 (1997)

[18] Yao Q, Yabuki SC, Mizutani FM, Sens. Acturators B., 65, 147 (2000)

[19] Mansouri A, Makris DP, Kefalas P, J. Pham. Bio. Anal., 39, 22 (2005)