Ion-exchange processing was employed to separate oligomers and lactic acid (LA) present in fermentation broths derived from apple pomace supplemented with yeast extract (YE) or corn steep liquor (CSL) using Lactobacillus rhamnosus. Experiments were carried out with Amberlite IRA 96 or Amberlite IRA 400 anion-exchange resins. Fermentation media supplemented with YE enabled higher oligosaccharide recovery yields than CSL-supplemented ones, whereas Amberlite IRA 96 provided a better separation of oligomers and LA than Amberlite IRA 400. Starting from a YE-supplemented fermentation medium containing 60.5 g of LA/L and 33.9 g of oligomers/L, column separation with Amberlite IRA 96 led to an effluent during the loading stage, which contained 17.9 g of oligosaccharides/L with an oligosaccharide/LA mass ratio of 90/10 g/g, whereas the regeneration stage provided a solution containing 30 g of LA/L. with a oligosaccharide/LA mass ratio of 8/92 g/g. Material balances showed that the regeneration effluent contained about 55% of the LA present in the feed stream, whereas only 2.1% was eluted jointly with oligomers during the loading phase.