A single-blow transient test technique based on axial dispersion model is proposed for the determination of both heat transfer coefficient and axial dispersion coefficient in plate heat exchangers, characterized by NTU and dispersive Peclet number respectively. The present experimental analysis deals with the effect of flow maldistribution on the transient temperature response for U-type plate heat exchangers. The experiments are carried out with uniform and non-uniform flow distributions for various flow rates and two different numbers of plates. Special effort has been made to differentiate the deviation from plug flow due to flow maldistribution and fluid backmixing. The fluid axial dispersion is used to characterize the backmixing and other deviations from plug flow. Due to unequal distribution of the fluid, the velocity of the fluid varies from channel to channel and hence the heat transfer coefficient variations are also taken into consideration. The computed outlet fluid temperatures are compared with experimental outlet temperatures, and the values of dispersive Peclet numbers are estimated. The results indicate that in order to get parameters independent of the number of plate used in single-blow experiment, it is essential to isolate flow maldistribution from backmixing. This paper has brought out a practical way in which this isolation can be done in the process of data reduction through suitable computational model. (C) 2009 Elsevier Ltd. All rights reserved.