The poly(2-methacryloyloxyethyl phosphorylcholine)-block-poly(D,L-lactide) (PMPC-b-PLA) was specially designed to develop biomimetic giant vesicles (GVs) and giant large compound vesicles via a simple spontaneous assemble in aqueous solution. The weight fraction of the hydrophilic PMPC block (f(PC)) was proved to play an important role in the size and morphology control of the self-assembled aggregates. The GVs with controlled micrometer size and biomimetic PMPC corona have great potential as artificial cell models. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci.118: 3197-3202, 2010