The preparation of honeycomb-patterned microporous films from a soluble fluorinated poly(siloxane imide) segmented copolymer (PSI) by means of water-droplet templating is reported first in this article. The fluorinated PSI was synthesized from 4,4'-(hexafluoroisopropylidene)diphthalic anhydride, 2,2-bis[4-(4-aminophenoxy)phenyl]hexafluoropropane, and diamine-terminated poly(dimethyl siloxane) by condensation polymerization. The obtained copolymer had good solubility in chlorinated solvents (chloroform, dichloromethane, and 1,2-dichloroethane), good thermal stability, and a microphase-separated amorphous structure. The effects of the copolymer concentration, atmospheric humidity, and solvent properties on the pat-tern formation were investigated. The results show that the film fabricated from the copolymer solution with chloroform as the solvent at a humidity of 90% and a concentration of 0.5 g/L had the most regular honeycomb-patterned micropores. We could tailor the pore shape and size by changing the copolymer concentration or the atmospheric humidity. The prepared regular honeycomb-patterned microporous PSI films have potential applications in cell culture and tissue engineering. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 119: 3329-3337, 2011