Statistical methodology for optimizing the dilute acid hydrolysis of sugarcane bagasse

Byung-Hwan Um; Sung-Ho Bae

Korean Journal of Chemical Engineering, Vol.28, No.5, 1172-1176, May, 2011

DOI10.1007/s11814-011-0058-9 Export Citation

Statistical methodology for optimizing the dilute acid hydrolysis of sugarcane bagasse

Byung-Hwan Um, and Sung-Ho Bae^{1, †}

Department of Chemical Engineering, Widener University, Chester, PA 19013, USA
¹Korea Institute of Energy Technology Evaluation & Planning, 997-10 Daechi-dong, Gangnam-gu, Seoul 135-280, Korea

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In converting advanced biomass to fuel, one pretreatment that has been extensively explored is a high temperature, dilute-sulfuric acid (H2SO4) process. This effectively hydrolyzes the hemicellulosic portion of the lignocellulosic biomass to fermentable sugars. Our aim was to optimize the concentration of sulfuric acid and residence time to release xylose from the hemicellulose of sugarcane bagasse. According to response surface methodology (RSM), the optimum concentrations and residence time were determined. The experimental maximum yield for xylose production was found to be 78.9% at 170℃ , 0.24% acid, for 15 min, and 76.4% at 200 ℃, 0.22% acid, for 6 min. The predicted maximum yield obtained for the fitted model was found to be 80.3% and 78.1% for the conditions stated above, respectively. The experimental yield was around 1.5% lower than that of the predicted yield. It was confirmed in this study that pentose sugars (xylose and arabinose) derived from hemicellulose fraction were further degraded. The statistical optimization method, which incorporates reaction time, temperature and acid concentration, did prove to provide a useful means of trading off the combined effects of these three variables on total xylose recovery yields.

Keywords:Sugarcane Bagasse;Pretreatment;Sulfuric Acid;Response Surface Methodology

[References]

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Ferrara MA, Bon EPS, Neto JSA, Appl. Biochem. Biotechnol., 98, 289 (2002)
Takahashi CM, Lima KGC, Takahashi DF, Alterthum F, World J. Microb. Biotechnol., 16, 829 (2000)
Frollini E, Pimenta MJA, Anais Assoc. Bras. Quim., 46(1), 43 (1997)
Pessoa Jr A, Mancilha IM, Sato S, Brazilian J. Chem. Eng., 14, 291 (1997)
Um BH, Frideman B, van Walsum GP, Holzforschung., 65, 51 (2011)
Baek SW, Kim JS, Park YK, Kim YS, Oh KK, Biotechnol. Biopro. Eng., 13, 332 (2008)
Um BH, van Walsum GP, Appl. Biochem. Biotechnol., 153(1-2), 127 (2009)
Jacobsen SE, Wyman CE, Appl. Biochem. Biotechnol., 84, 81 (2000)
Walton S, van Walsum GP, van Heiningen A, Bioresour. Technol., 101(6), 1935 (2009)
Um BH, van Walsum GP, Bioresour. Technol., 101, 5978 (2010)
Chen HC, Food Biotechnol., 10, 13 (1996)
Giovanni M, Food Technol., 37, 96 (1983)
Cordenunsi BR, da Silva RSF, Srivastava KC, Fabre-Sanches S, Perre MA, J. Biotechnol., 2, 1 (1985)
Rao PV, Jayaraman K, Lakshmanan CM, Process Biochem., 28, 391 (1993)
Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D, Determination of Sugars, Byproduct, and Degradation Products in Liquid Fraction Process Samples, NREL/TP-510-42623 (2008)
Kim SB, Um BH, Park SC, Appl. Biochem. Biotechnol., 91, 91 (2001)
Um BH, Karim MN, Henk LL, Appl. Biochem. Biotechnol., 105, 115 (2002)
Um BH, Hanley TR, Korean J. Chem. Eng., 25(5), 1094 (2008)
Um BH, van Walsum GP, Appl. Biochem. Biotechnol., 161(1-8), 432 (2010)
Sipos B, Reczey J, Somorai Z, Kadar Z, Dienes D, Reczey K, Appl. Biochem. Biotechnol., 153(1-23), 151 (2008)
Jeong TS, Um BH, Kim JS, Oh KK, Appl. Biochem. Biotechnol., 161(1-8), 22 (2010)
Lavarack BP, Griffin GJ, Rodman D, Biomass Bioenerg., 23(5), 367 (2002)

[Referenced By]

[1] Pandey A, Soccol CR, Nigam P, Soccol VT, Bioresour. Technol., 74(1), 69 (2000)

[2] Ferrara MA, Bon EPS, Neto JSA, Appl. Biochem. Biotechnol., 98, 289 (2002)

[3] Takahashi CM, Lima KGC, Takahashi DF, Alterthum F, World J. Microb. Biotechnol., 16, 829 (2000)

[4] Frollini E, Pimenta MJA, Anais Assoc. Bras. Quim., 46(1), 43 (1997)

[5] Pessoa Jr A, Mancilha IM, Sato S, Brazilian J. Chem. Eng., 14, 291 (1997)

[6] Um BH, Frideman B, van Walsum GP, Holzforschung., 65, 51 (2011)

[7] Baek SW, Kim JS, Park YK, Kim YS, Oh KK, Biotechnol. Biopro. Eng., 13, 332 (2008)

[8] Um BH, van Walsum GP, Appl. Biochem. Biotechnol., 153(1-2), 127 (2009)

[9] Jacobsen SE, Wyman CE, Appl. Biochem. Biotechnol., 84, 81 (2000)

[10] Walton S, van Walsum GP, van Heiningen A, Bioresour. Technol., 101(6), 1935 (2009)

[11] Um BH, van Walsum GP, Bioresour. Technol., 101, 5978 (2010)

[12] Chen HC, Food Biotechnol., 10, 13 (1996)

[13] Giovanni M, Food Technol., 37, 96 (1983)

[14] Cordenunsi BR, da Silva RSF, Srivastava KC, Fabre-Sanches S, Perre MA, J. Biotechnol., 2, 1 (1985)

[15] Rao PV, Jayaraman K, Lakshmanan CM, Process Biochem., 28, 391 (1993)

[16] Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D, Determination of Sugars, Byproduct, and Degradation Products in Liquid Fraction Process Samples, NREL/TP-510-42623 (2008)

[17] Kim SB, Um BH, Park SC, Appl. Biochem. Biotechnol., 91, 91 (2001)

[18] Um BH, Karim MN, Henk LL, Appl. Biochem. Biotechnol., 105, 115 (2002)

[19] Um BH, Hanley TR, Korean J. Chem. Eng., 25(5), 1094 (2008)

[20] Um BH, van Walsum GP, Appl. Biochem. Biotechnol., 161(1-8), 432 (2010)

[21] Sipos B, Reczey J, Somorai Z, Kadar Z, Dienes D, Reczey K, Appl. Biochem. Biotechnol., 153(1-23), 151 (2008)

[22] Jeong TS, Um BH, Kim JS, Oh KK, Appl. Biochem. Biotechnol., 161(1-8), 22 (2010)

[23] Lavarack BP, Griffin GJ, Rodman D, Biomass Bioenerg., 23(5), 367 (2002)