Korean Journal of Chemical Engineering, Vol.13, No.3, 266-274, May, 1996
KINETIC MODEL FOR EFFECTS OF ETHANOL AND PHOSPHATE ON CELL GROWTH AND EMULSAN PRODUCTION IN ACINETOBACTER CALCOACETICUS RAG-1
A mathematical kinetic model was proposed to describe the cell growth and the emulsan production in batch cultivations of Acinetobacter calcoaceticus RAG-1. Ethanol and phosphate concentrations were chosen as the dey variables, which affected the cell growth and emulsan production in the batch cultivations. The cell growth was inhilbited by high concentrations of ethanol and was slightly affected by intracellular phosphate level. And the emulsan production was related to the intracellular phosphate level dependent upon the extracellular phosphate concentration. Kinetic model for the cell growth was formulated using the ethanol inhibition term and the intracellular phosphate level. The re-lationship between extracellular and intracellular phosphate level was expressed by the concept of active transport. Kinetic model for the emulsan production was represented using growth-associated term and intracellular phosphate inhilbition term. Release of emulsan was presumed as the primary release from the surface of viable cell and the secondary release by cell lysis. The model predicted the experimental results with good agreement.
Keywords:Emulsan;Acinetobacter calcoaceticus RAG-1;Kinetic Model;Ethanol Inhibition;Phosphate Effect
- Abbott BJ, J. Gen. Microbiol., 75, 383 (1973)
- Abbott BJ, Laskin AI, McCoy CJ, Appl. Microbiol., 25, 787 (1973)
- Bramble JL, Graves DJ, Brodelius P, Biotechnol. Bioeng., 37, 859 (1991)
- Choi JW, Choi HG, Lee WH, J. Biotechnol., 45, 217 (1996)
- Choi JW, Choi HG, Lee KS, Lee WH, J. Biotechnol., in press (1996)
- Dubois M, Gillies KA, Hamilton JK, Rebers PA, Smith F, Anal. Chem., 28, 350 (1959)
- Gutnick DL, Bayer EA, Rubinowitz C, Pines O, Shabtai Y, Goldman S, Rosenberg E, "Emulsan Production in Acinetobacter RAG-1," Proceeding of the Sixth International Fermentation Symposium, Advances in Biotechnology, Vol. 3, Fermantation Product, Moo-Young, M., Robinson, C.W. and Vezina, C., eds., Pergamon Press, Toronto (1980)
- Gutnick DL, Shabtai Y, "Exopolysaccharide Bioemulsifier," Biosurfactants and Biotechnology, Kosaric, N., Caims, W.L. and Gray, N.C.C., eds., Marcel Dekker, Inc., New York (1987)
- IBM Corporation, Technical Publications Department, "System/360 Scientific Subroutine Package Programmer's Manual," 5th ed., New York (1970)
- Jarman TR, Pace GW, Arch. Microbiol., 137, 231 (1984)
- Kosaric N, Caims WL, Gary NCC, "Biotechnology and Surfactant Industry," Biosurfactants and Biotechnology, Kosaric, N., Caims, W.L. and Gray, N.C.C., eds., Marcel Dekker, Inc., New York (1987)
- Luong JHT, Biotechnol. Bioeng., 29, 242 (1987)
- Maron MJ, "Numerical Analysis: A Practical Approach," Macmillan Publishing Co., New York (1982)
- Metzler CM, Elfring GL, McEwen AJ, Biometric., 30, 562 (1974)
- Mulchandani A, Luong JHT, Enzyme Microb. Technol., 11, 66 (1989)
- Pazoutova S, Votruba J, Rehacek Z, Biotechnol. Bioeng., 23, 2837 (1981)
- Reisfeld A, Rosenberg E, Gutnick DL, Appl. Microbiol., 24, 363 (1972)
- Rosenberg E, Zuckerberg A, Rubinowitz C, Gutnick DL, Appl. Environ. Microbiol., 37, 402 (1979)
- Rosenberg E, Perry A, Gibson DT, Gutnick DL, Appl. Environ. Microbiol., 37, 409 (1979)
- Rosenberg E, Zosim Z, Belsky I, Gutnick DL, "Interaction of Acinetobacter RAG-1 Emulsan with Hydrocarbon," Proceeding of the Sixth International Fermantation Symposium, Advances in Biotechnology, Vol. 3, Fermentaton Product, Moo-Young, M., Robinson, C.W. and Vezina, C., eds., Pergamon Press, Toronto (1980)
- Schaefer E, "Synthesis and Release of Emulsan by Acinetobacter Calcoaceticus," Ph.D. Thesis, Massachusetts Institute of Technology, Cambridge, MA (1985)
- Sutherland IW, Norval M, Biochem. J., 120, 567 (1970)
- Toda K, Yabe I, Biotechnol. Bioeng., 21, 487 (1979)
- Troy FA, Frerman FE, Heath EC, J. Biol. Chem., 246, 118 (1971)
- Wang SD, Wang DIC, Biotechnol. Bioeng., 34, 1261 (1989)
- Zuckerberg A, Diver A, Perry Z, Gutnick DL, Rosenberg E, Appl. Environ. Microbiol., 37, 414 (1979)