This paper presents a novel electroenzymatic method for the treatment of bisphenol A (BPA) in a membraneless electrochemical reactor. The electrochemical reactor was arranged with a stainless steel and an enzymatic film as anode and cathode, respectively. The enzymatic film was formed by immobilizing hemoglobin (Hb) on carbon fiber. In the membraneless electrochemical reactor, hydrogen peroxide (H2O2) was generated in situ in cathode and BPA was oxidated and removed by the combining Hb with H2O2. The experimental conditions for electrogeneration of H2O2 and electroremoval of BPA were optimized. Experimental results showed that in supplied voltage 2.4 V. pH 5.0 and oxygen flow rate 25 mL/min, the electrogeneration of H2O2 and the electroenzymatic removal of BPA were highest. Under optimal operation conditions, the removal efficiency of BPA reached 50.7% in 120 min and then kept constant when further prolonging the period of reaction. Compared with electrochemical and biochemical methods, the removal of BPA through electroenzymatic method was comparatively favorable. (C) 2010 Elsevier B.V. All rights reserved.