Unrefined chemical pulps of bleached and unbleached softwood (Scots pine, Pinus sylvestris) and hardwood (Eucalyptus, Eucalyptus globulus) were subjected to indentation tests using a nanoindenter equipped with an AFM scanner. Tests on unbleached pulps revealed no difference in hardness values between softwood and hardwood, but bleaching treatment decreased the hardness values of both pulps. Indentation modulus of 12 GPa was observed for unbleached softwood pulps, which is 25% higher than unbleached hardwood pulps. Bleaching treatment again decreased the indentation modulus of the softwood pulps, whereas it slightly increased the indentation modulus of the hardwood pulps. After bleaching and drying processes, only negligible difference was observed in cell wall mechanical properties (hardness and indentation modulus) between hardwood and softwood pulps. This study is based on latewood pulp fibres.