Post-Translational His-Cys Cross-Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species

Fujieda N; Ikeda T; Murata M; Yanagisawa S; Aono S; Ohkubo K; Nagao S; Ogura T; Hirota S; Fukuzumi S; Nakamura Y; Hata Y; Itoh S

Journal of the American Chemical Society, Vol.133, No.5, 1180-1183, 2011

Fujieda N, Ikeda T, Murata M, Yanagisawa S, Aono S, Ohkubo K, Nagao S, Ogura T, Hirota S, Fukuzumi S, Nakamura Y, Hata Y, Itoh S

Autocatalytic formation of His-Cys cross-linkage in the enzyme active site of tyrosinase from Aspergillus oryzae has been demonstrated to proceed by the treatment of apoenzyme with Cu-II under aerobic conditions, where a (mu-eta(2):eta(2)-peroxo)dicopper(II) species has been suggested to be involved as a key reactive intermediate.