화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.35, No.3, 216-222, May, 2011
Export Citation
방사선 그래프트법에 의해 제조된 탄소나노튜브 지지체를 기반으로 한 전기화학 미생물 바이오센서의 제작
Fabrication of Electrochemical Microbial Biosensor Based on MWNT Supports Prepared by Radiation-Induced Graft Polymerization
신수란, 권해두, 최성호†
  • 한남대학교 화학과
Soo-Ran Shin, Hai-Doo Kwen, and Seong-Ho Choi†
  • Department of Chemistry, Hannam University, 461-6 Yuseong-gu, Jeonmin-dong, Daejeon 305-811, Korea
E-mail:
초록
4급 아민에 의한 이온성 및 3급 아민에 의한 비공유전자쌍의 이중 특성을 갖은 다중벽 탄소나노튜브 지지체를 글리시딜 메타크릴레이트의 방사선 그래프트법을 수행한 후, 아민화 반응을 수행하여 제조하였다. 제조된 이중 다중벽 탄소나노튜브 지지체와 나피온 용액을 혼합 후, 이 코팅용액을 GC 전극 표면에 코팅시킨 후, 여기에 미생물인 Alkaligenes spp.를 고정화하여 미생물 바이오센서를 제작하였다. 이 미생물 센서의 페놀에 대한 검출범위는 0.005∼7.0 mM이었다. 이 미생물 바이오센서를 이용하여 상용의 적포도주에서 페놀함량을 측정하였다.
A multi-walled carbon nanotube (MWNT) support with dual properties, an ionic property via tetra-amine and unpaired electrons via tri-amine, was prepared by radiation-induced graft polymerization of glycidyl methacrylate (GMA) and the subsequent amination of its epoxy group. The electrochemical microbial biosensor (EMB) was then fabricated by immobilization of a microbe (Alkaligenes spp.) onto the dual property-modified electrode, which was prepared with the mixture of the MWNT support and a Nafion® solution on a glass carbon (GC) electrode surface by a hand-casting method. The sensing range of the prepared EMB for phenol in a phosphate buffer solution was 0.005∼7.0 mM. The total concentration of phenolic compounds in a commercial red wine was also determined using the EMB.
Keywords:radiation-induced graft polymerization;electrochemical microbial biosensor;MWNT supports;amination.
  1. Amin GA, Al-Talhi A, World Appl. Sci. J., 2, 62 (2007)
  2. Deo YM, Gaucher GM, Biotechnol. Bioeng., 26, 285 (2004)
  3. Sauer M, Porro D, Mattanovich D, Branduardi P, Trends Biotechnol., 26, 100 (2008)
  4. Jenkins RL, Wilson EM, Angus RA, Howell WM, Kirk M, Moore R, Nance M, Brown A, Environ. Health Perspect., 112, 1508 (2004)
  5. Kapoor M, Nair LM, Kuhad RC, Biochem. Eng. J., 38, 88 (2008)
  6. Reshetilov AN, Trotsenko JA, Morozova NO, Iliasov PV, Ashin VV, Process Biochem., 36, 1015 (2001)
  7. Karube I, Nakanishi K, IEEE Eng. Med. Biol., 13, 364 (1994)
  8. Svitel J, Tkac J, Vostiar I, Navratil M, Stefuca V, Bucko M, Gemeiner P, Biotechnol. Lett., 28(24), 2003 (2006)
  9. Tkac J, Stefuca V, Gemeiner P, Applications of Cell Immobilisation Biotechnology, Nedovic V, Wilaert, R Editors, Springer, Netherlands, Part 5, pp. 549-566 (2005)
  10. Timur S, Haghighi B, Tkac J, Pazarlıo˘glu N, Telefoncu A, Gorton L, Bioelectrochemistry., 71, 215 (2006)
  11. Wang J, Electroanalysis., 17, 7 (2005)
  12. Wang SG, Zhang Q, Wang RL, Yoon SF, Biochem. Biophys. Res. Commun., 311(3), 572 (2003)
  13. Merkoc A, Pumera M, Llopis X, Perez B, Valle M, Alegret S, Trend Anal. Chem., 24, 826 (2003)
  14. Wang J, Musameh M, Lin YH, J. Am. Chem. Soc., 125(9), 2408 (2003)
  15. Yan A, Bussche AVD, Kane AB, Hurt RH, Carbon., 45, 2463 (2007)
  16. Wu Z, Feng W, Feng Y, Liu Q, Xu X, Sekino T, Fujii A, Ozaki M, Carbon., 45, 1212 (2007)
  17. Star A, Stoddart JF, Steuerman D, Diehl M, Boukai A, Wong EW, Yang X, Chung SW, Choi H, Angew JRH, Chem. Int. Ed., 40, 1721 (2001)
  18. Choi SH, Park SY, Nho YC, Radiat. Phys. Chem., 57, 179 (2000)
  19. Choi SH, Nho YC, Radiat. Phys. Chem., 58, 157 (2000)
  20. Choi SH, Lee KP, Lee JG, Nho YC, J. Appl. Polym. Sci., 77(3), 500 (2000)
  21. Choi SH, Nho YC, J. M. S., Pure Appl. Chem., A37, 1053 (2000)
  22. Choi SH, Lee KP, Lee JG, Microchem. J., 68, 205 (2001)
  23. Lee KP, Kang HJ, Joo DR, Choi SH, Radiat. Phys. Chem., 60, 473 (2001)
  24. Piao MH, Yang DS, Yoon KR, Lee S, Choi SH, Sensors., 9, 1662 (2009)
  25. Kim KI, Kang HY, Lee JC, Choi SH, Sensors., 9, 6701 (2009)
  26. Campanella L, Bonanni A, Finotti E, Tomasetti M, Biosens. Bioelectron., 19, 641 (2004)
  27. Luximon-Ramma A, Bahorun T, Crozier A, Zbarsky V, Datla KP, Dexter DT, Food Res. Int., 38, 357 (2005)
  28. Yang DS, Jung DJ, Choi SH, Radiat. Phys. Chem., 79, 434 (2010)