An efficient separation process of flavonoid from Ginkgo extract was developed in this study. Polyamide resin offered the fine adsorption capacity, and its adsorption rate at 25 degrees C fitted well to the Langmuir isotherm. Dynamic adsorption and desorption experiments were conducted to optimize the separation process of total flavonoids from the Ginkgo extract. After one run, the content of total flavonoids increased from 24.0% to 55.0%. The method will provide a potential approach for large-scale separation and purification of flavonoid for its wide pharmaceutical use.