Preparation and Characterization of Alpha-Tricalcium Phosphate Cements Incorporated with Polyamino acids

Hwa Sung Lee; Soon Yong Kwon; Eun Mi Seo; Yong-Hee Kim; Sung Soo Kim

Macromolecular Research, Vol.19, No.1, 90-96, January, 2011

DOI10.1007/s13233-011-0113-8 Export Citation

Preparation and Characterization of Alpha-Tricalcium Phosphate Cements Incorporated with Polyamino acids

Hwa Sung Lee, Soon Yong Kwon , Eun Mi Seo¹, Yong-Hee Kim¹, and Sung Soo Kim^{2, †}

Department of Orthopedic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea
¹Department of Bioengineering, College of Engineering, Hanyang University, Seoul, 133-791, Korea
²Advanced Materials Division, Korea Research Institute of Chemical Technology, Daejon 305-600, Korea

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Polymeric calcium phosphate cements (PCPCs) derived from biodegradable polyamino acids, such as polyaspartic acid (PAA) and poly-γ-glutamic acid (γ-PGA), were prepared in an attempt to improve their cohesion strength and injectability. The characteristics of the calcium phosphate cements (CPCs), such as dynamic viscosity, paste injectability, initial setting time, compressive strength and cell compatibility, were assessed. The dynamic viscosity increased significantly with γ-PGA incorporation but decreased with PAA incorporation. The injectability increased with the concentration of polyamino acids and that of the CPCs mixed with γ-PGA reached 94%. The initial setting time decreased with the γ-PGA concentration in the cement liquid and reached approximately 10-15 min when the γ-PGA concentration was 1-2 wt%. The compressive strength (CS) of CPCs after 1 week-incubation decreased with the incorporation of PAA and γ-PGA. γ-PGA incorporation deteriorated the CS more than PAA incorporation because the dissolution of alpha-tricalcium phosphate (α-TCP) was retarded. Saos-2 cells grown on CPCs with polyamino acids spread more than those grown on the CPC without them.

Keywords:bone cement;calcium phosphate;polyaspartic acid;polyamino acid;biomaterial.

[References]

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Chohayeb AA, Chow LC, Tsaknis PJ, J. Endod., 13, 384 (1987)
Sugawara A, Chow LC, Takagi S, Chohayeb H, J. Endod., 16, 162 (1990)
Bohner M, Gbureck U, Barralet JE, Biomaterials, 26, 6423 (2005)
Cho YR, Gosain AK, Clin. Plast. Surg., 31, 377 (2004)
Kveton JF, Friedman CD, Constantino PD, Am. J. Otol., 16, 465 (1995)
Ishikawa K, Eanes ED, J. Dent. Res., 72, 474 (1993)
Liu C, Shen W, Gu Y, Hu L, J. Biomed. Mater. Res., 35, 75 (1997)
Li Y, Zhang X, de Groot K, Biomaterials, 18, 737 (1997)
Khairoun I, Driessens FCM, Boltong MG, Planell JA, Wenz R, Biomaterials, 20, 393 (1999)
Wang X, Chen L, Xiang H, Ye J, J. Biomed. Mater. Res., 81B, 410 (2007)
Cherng A, Takagi S, Chow LC, J. Biomed. Mater. Res., 35, 273 (1997)
Chen GX, Li WW, Yu XM, Sun K, J. Mater. Sci., 44(3), 828 (2009)
Kim SS, Seo M, Chung JW, Kwon SY, Kim YS, J. Appl. Polym. Sci., 113(2), 1223 (2009)
Ginebra MP, Rilliard A, Ferenandez E, Elvira C, Roman JS, Planell JA, J. Biomed. Mater. Res., 57, 113 (2001)
Gbureck U, Barralet JE, Spatz K, Grover LM, Thull R, Biomaterials, 25, 2187 (2004)
Chen GX, Li WW, Yu XM, Sun K, J. Mater. Sci., 44(3), 828 (2009)
Chow LC, Dent. Mater., 28, 1 (2009)
Tenhuisen KS, Brown PW, J. Mater. Sci. -Mater. Med., 5, 291 (1994)
Bigi A, Boanini E, Botter R, Panzavolta S, Rubini K, Biomaterials, 23, 1849 (2002)
Matsuya Y, Antonucci JM, Matsuya S, Takagi S, Chow LC, Dent. Mater., 12, 2 (1996)
Miyazaki K, Horibe T, Chow LC, Takagi S, Antonucci JM, Dent. Mater., 9, 41 (1993)
Matsuya S, Takagi S, Chow LC, J. Mater. Sci., 31(12), 3263 (1996)

[Referenced By]

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[1] Brown WE, Chow IC, J. Dent. Res., 62, 672 (1983)

[2] Chohayeb AA, Chow LC, Tsaknis PJ, J. Endod., 13, 384 (1987)

[3] Sugawara A, Chow LC, Takagi S, Chohayeb H, J. Endod., 16, 162 (1990)

[4] Bohner M, Gbureck U, Barralet JE, Biomaterials, 26, 6423 (2005)

[5] Cho YR, Gosain AK, Clin. Plast. Surg., 31, 377 (2004)

[6] Kveton JF, Friedman CD, Constantino PD, Am. J. Otol., 16, 465 (1995)

[7] Ishikawa K, Eanes ED, J. Dent. Res., 72, 474 (1993)

[8] Liu C, Shen W, Gu Y, Hu L, J. Biomed. Mater. Res., 35, 75 (1997)

[9] Li Y, Zhang X, de Groot K, Biomaterials, 18, 737 (1997)

[10] Khairoun I, Driessens FCM, Boltong MG, Planell JA, Wenz R, Biomaterials, 20, 393 (1999)

[11] Wang X, Chen L, Xiang H, Ye J, J. Biomed. Mater. Res., 81B, 410 (2007)

[12] Cherng A, Takagi S, Chow LC, J. Biomed. Mater. Res., 35, 273 (1997)

[13] Chen GX, Li WW, Yu XM, Sun K, J. Mater. Sci., 44(3), 828 (2009)

[14] Kim SS, Seo M, Chung JW, Kwon SY, Kim YS, J. Appl. Polym. Sci., 113(2), 1223 (2009)

[15] Ginebra MP, Rilliard A, Ferenandez E, Elvira C, Roman JS, Planell JA, J. Biomed. Mater. Res., 57, 113 (2001)

[16] Gbureck U, Barralet JE, Spatz K, Grover LM, Thull R, Biomaterials, 25, 2187 (2004)

[17] Chen GX, Li WW, Yu XM, Sun K, J. Mater. Sci., 44(3), 828 (2009)

[18] Chow LC, Dent. Mater., 28, 1 (2009)

[19] Tenhuisen KS, Brown PW, J. Mater. Sci. -Mater. Med., 5, 291 (1994)

[20] Bigi A, Boanini E, Botter R, Panzavolta S, Rubini K, Biomaterials, 23, 1849 (2002)

[21] Matsuya Y, Antonucci JM, Matsuya S, Takagi S, Chow LC, Dent. Mater., 12, 2 (1996)

[22] Miyazaki K, Horibe T, Chow LC, Takagi S, Antonucci JM, Dent. Mater., 9, 41 (1993)

[23] Matsuya S, Takagi S, Chow LC, J. Mater. Sci., 31(12), 3263 (1996)