Control of Adhesion, Focal Adhesion Assembly, and Differentiation of Myoblasts by Enzymatically Crosslinked Cell-Interactive Hydrogels

Indong Jun; Kyung Min Park; Dong Yun Lee; Ki Dong Park; Heungsoo Shin

Macromolecular Research, Vol.19, No.9, 911-920, September, 2011

DOI10.1007/s13233-011-0909-6 Export Citation

Control of Adhesion, Focal Adhesion Assembly, and Differentiation of Myoblasts by Enzymatically Crosslinked Cell-Interactive Hydrogels

Indong Jun¹, Kyung Min Park², Dong Yun Lee¹, Ki Dong Park², and Heungsoo Shin^{1, 3, †}

¹Department of Bioengineering, Hanyang University, Seoul, 133-791, Korea
²Department of Molecular Science and Technology, Ajou University, Gyeonggi, 443-749, Korea
³Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul, 133-791, Korea

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The development of an artificial matrix is critical as both a substrate to control the cell behavior and as a tool for examining the roles of cellular microenvironment in biology. This study developed cell-interactive hydrogels containing a Arg-Gly-Asp (RGD) peptide, cross-linked via bio-inspired enzymatic processes using H2O2 and horseradish peroxidase (HRP) as the initiators and examined how they controlled myoblast functions. The cell-interactive hydrogels modulated the adhesion and proliferation of myoblast, depending on the peptide density. Furthermore, the expression of focal adhesion proteins and myogenic differentiation were up-regulated significantly in myoblasts cultured on peptide-incorporated hydrogels. Therefore, the novel hydrogel system can be used to regulate the cell function for many tissue engineering applications.

Keywords:in situ gelatin;enzyme polyerization;RGD peptide;myoblast adhesion;myogenic differentiation.

[References]

Shin H, Jo S, Mikos AG, Biomaterials, 24, 4353 (2003)
Lutolf MP, Hubbell JA, Nat. Biotechnol., 23, 47 (2005)
Shin YM, Kim KS, Lim YM, Nho YC, Shin H, Biomacromolecules, 9(7), 1772 (2008)
Robertus J, Browne WR, Feringa BL, Chem. Soc. Rev., 39, 354 (2010)
Lutolf MP, Integr. Biol., 1, 235 (2009)
Jun I, Jeong S, Shin H, Biomaterials, 30, 2038 (2009)
Engler AJ, Sen S, Sweeney HL, Discher DE, Cell, 126, 677 (2006)
Nakamura M, Yamaguchi K, Mie M, Nakamura M, Akita K, Kobatake E, Bioconjugate Chem., 20, 1759 (2009)
Dang JM, Leong KW, Adv. Mater., 19(19), 2775 (2007)
Lutolf MR, Weber FE, Schmoekel HG, Schense JC, Kohler T, Muller R, Hubbell JA, Nat. Biotechnol., 21, 513 (2003)
Phelps EA, Landazuri N, Thule PM, Taylor WR, Garcia AJ, Proc. Natl. Acad. Sci. U.S.A., 107, 3323 (2010)
Wang LS, Chung JE, Chan PPY, Kurisawa M, Biomaterials, 31, 1148 (2010)
DeForest CA, Polizzotti BD, Anseth KS, Nat. Mater., 8(8), 659 (2009)
Benoit DSW, Schwartz MP, Durney AR, Anseth KS, Nat. Mater., 7(10), 816 (2008)
Evangelista MB, Hsiong SX, Fernandes R, Sampaio P, Kong HJ, Barrias CC, Salema R, Barbosa MA, Mooney DJ, Granja PL, Biomaterials, 28, 3644 (2007)
Shin H, Zygourakis K, Farach-Carson MC, Yaszemski MJ, Mikos AG, Biomaterials, 25, 895 (2004)
Lee F, Chung JE, Kurisawa M, J. Control. Release, 134, 186 (2009)
Jin R, Teixeira LSM, Dijkstra PJ, van Blitterswijk CA, Karperien M, Feijen J, Biomaterials, 31, 3103 (2010)
Park KM, Shin YM, Joung YK, Shin H, Park KD, Biomacromolecules, 11(3), 706 (2010)
Wang LS, Boulaire J, Chan PP, Chung JE, Kurisawa M, Biomaterials, 31, 8608 (2010)
Bershadsky AD, Balaban NQ, Geiger B, Annu. Rev. Cell Dev. Biol., 19, 677 (2003)
Buxboim A, Ivanovska IL, Discher DE, J. Cell Sci., 123, 297 (2010)
Gao L, McBeath R, Chen CS, Stem Cells, 28, 564 (2010)
Yim EKF, Darling EM, Kulangara K, Guilak F, Leong KW, Biomaterials, 31, 1299 (2010)
Clemente CFMZ, Corat MAF, Saad STO, Franchini KG, Am. J. Physiol. Regul. Integr. Comp. Physiol., 289, R862 (2005)
Dedieu S, Poussard S, Mazeres G, Grise F, Dargelos E, Cottin P, Brustis JJ, Exp. Cell Res., 292, 187 (2004)
Park KM, Jun I, Joung YK, Shin H, Park KD, Soft Matter, 7, 986 (2011)
Deshmukh M, Singh Y, Gunaseelan S, Gao D, Stein S, Sinko PJ, Biomaterials, 31, 6675 (2010)
Kurisawa M, Chung JE, Yang YY, Gao SJ, Uyama H, Chem. Commun., 4312 (2005)
Yamaki K, Harada I, Goto M, Cho CS, Akaike T, Biomaterials, 30, 1421 (2009)
Rowley JA, Mooney DJ, J. Biomed. Mater. Res., 60, 217 (2002)
Yeo Y, Geng WL, Ito T, Kohane DS, Burdick JA, Radisic M, J. Biomed. Mater. Res. Part B: Appl. Biomater., 81B, 312 (2007)
Connelly JT, Garcia AJ, Levenston ME, Biomaterials, 28, 1071 (2007)
Bacakova L, Filova E, Rypacek F, Svorcik V, Stary V, Physiol. Res., 53, S35 (2004)
Keselowsky BG, Garcia AJ, Biomaterials, 26, 413 (2005)
Chung IM, Enemchukwu NO, Khaja SD, Murthy N, Mantalaris A, Garcia AJ, Biomaterials, 29, 2637 (2008)
LaNasa SM, Bryant SJ, Acta Biomater., 5, 2929 (2009)
Charge SBP, Rudnicki MA, Physiol. Rev., 84, 209 (2004)
Le Grand F, Rudnicki MA, Curr. Opin. Cell Biol., 19, 628 (2007)
Andres V, Walsh K, J. Cell Biol., 132, 657 (1996)
Gersbach CA, Byers BA, Pavlath GK, Guldberg RE, Garcia AJ, Biotechnol. Bioeng., 88(3), 369 (2004)
Chandran R, Knobloch TJ, Anghelina M, Agarwal S, Am. J. Physiol. Cell Physiol., 293, C267 (2007)

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[4] Robertus J, Browne WR, Feringa BL, Chem. Soc. Rev., 39, 354 (2010)

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[10] Lutolf MR, Weber FE, Schmoekel HG, Schense JC, Kohler T, Muller R, Hubbell JA, Nat. Biotechnol., 21, 513 (2003)

[11] Phelps EA, Landazuri N, Thule PM, Taylor WR, Garcia AJ, Proc. Natl. Acad. Sci. U.S.A., 107, 3323 (2010)

[12] Wang LS, Chung JE, Chan PPY, Kurisawa M, Biomaterials, 31, 1148 (2010)

[13] DeForest CA, Polizzotti BD, Anseth KS, Nat. Mater., 8(8), 659 (2009)

[14] Benoit DSW, Schwartz MP, Durney AR, Anseth KS, Nat. Mater., 7(10), 816 (2008)

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[19] Park KM, Shin YM, Joung YK, Shin H, Park KD, Biomacromolecules, 11(3), 706 (2010)

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[21] Bershadsky AD, Balaban NQ, Geiger B, Annu. Rev. Cell Dev. Biol., 19, 677 (2003)

[22] Buxboim A, Ivanovska IL, Discher DE, J. Cell Sci., 123, 297 (2010)

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[24] Yim EKF, Darling EM, Kulangara K, Guilak F, Leong KW, Biomaterials, 31, 1299 (2010)

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[40] Andres V, Walsh K, J. Cell Biol., 132, 657 (1996)

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