Protective effect of nicotinic acid on human albumin during UV-C irradiation

Antonio Zuorro; Roberto Lavecchia

Korean Journal of Chemical Engineering, Vol.28, No.10, 1965-1968, October, 2011

DOI10.1007/s11814-011-0083-8 Export Citation

Protective effect of nicotinic acid on human albumin during UV-C irradiation

Antonio Zuorro, and Roberto Lavecchia^†

Department of Chemical Engineering, La Sapienza University, Via Eudossiana 18, Rome 00184, Italy

E-mail:

The potential of nicotinic acid (NiAc), a vitamin B3 compound with antioxidant activity, to protect human albumin against the damage caused by exposure to 254-nm UV-C radiation was investigated. Experiments performed at irradiance levels of 3.5-15 W m^(-2) and application times between 5 and 30 min showed that albumin undergoes rapid dose-dependent aggregation when subjected to irradiation. Addition of 0.2 mM NiAc to the albumin solution resulted in a remarkable reduction of protein damage. Protection provided by NiAc was effective up to radiation doses of about 30 kJ m^(-2), which are significantly higher than those required for complete microbial and viral inactivation.

Keywords:Albumin;Nicotinic Acid;Sterilization;UV-C;Viral Inactivation

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[Referenced By]

[1] Quinlan GJ, Martin GS, Evans TW, Hepatology., 41, 1211 (2005)

[2] Matejtschuk P, Dash CH, Gascoigne EW, Br. J. Anaesth., 85, 887 (2000)

[3] Burnouf T, Radosevich M, Blood Rev., 14, 94 (2000)

[4] Klein HG, Bryant BJ, Vox Sang., 4, 154 (2009)

[5] Macleod AJ, Dev. Biol. (Basel)., 118, 139 (2004)

[6] Chin S, Jin R, Wang XL, Hamman J, Marx G, Mou X, Andersson I, Lindquist LO, Horowitz B, Photochem. Photobiol., 65, 432 (1997)

[7] Wang J, Mauser A, Chao SF, Remington K, Treckmann R, Kaiser K, Pifat D, Hotta J, Vox Sang., 86, 230 (2004)

[8] Li Q, MacDonald S, Bienek C, Foster PR, MacLeod AJ, Biologicals., 33, 101 (2005)

[9] Davies KJA, Delsignore ME, J. Biol. Chem., 262, 9908 (1987)

[10] Farmer JA, Curr. Atheroscler. Rep., 11, 87 (2009)

[11] Sanyal S, Karas RH, Kuvin JT, Expert Opin. Pharmacother., 8, 1711 (2007)

[12] Hu ML, Chen YK, Lin YF, Chem.-Biol. Interact., 97, 63 (1995)

[13] Bosse D, Praus M, Kiessling P, Nyman L, Andresen C, Waters J, Schindel F, J. Clin. Pharmacol., 45, 57 (2005)

[14] Michnik A, Michalik K, Drzazga Z, J. Photochem. Photobiol., 90, 170 (2008)

[15] Artyukhov VG, Pantyavin AA, Vashanov GA, J. Appl. Spectrosc., 68, 291 (2001)

[16] Oliva A, Santovena A, Llabres M, Farina JB, J. Pharm. Pharmacol., 51, 385 (1999)

[17] Kerwin BA, Remmele RL, J. Pharm. Sci., 96, 1468 (2007)

[18] Stadtman ER, Free Radical Res., 40, 1250 (2006)

[19] Naskalski JW, Bartosz G, Adv. Clin. Chem., 35, 161 (2001)

[20] Cioci F, Lavecchia R, Chem. Biochem. Eng. Q., 12, 191 (1998)

[21] Su R, Qi W, He Z, Zhang Y, Jin F, Food Hydrocolloids., 22, 995 (2008)

[22] Chin S, Williams B, Gottlieb P, Margolis-Nunno H, Ben-Hur E, Hamman J, Jin R, Dubovi E, Horovitz B, Blood., 86, 4331 (1995)

[23] Marx G, Mou X, Freed R, Ben-Hur E, Yang C, Horowitz B, Photochem. Photobiol., 63, 51 (1996)

[24] Ben-Hur E, Horowitz B, Photochem. Photobiol., 62, 383 (1995)